My data is usually represented as 3D data (stack of several 2D fluorescent images, each with constant displacement along optical axis from the other), common voxel size is 100*100*300 nanometer. How can I understand how blurry the image is? For 2D images visual information fidelity metric (VIF) and multi-scale structural similarity index (MS-SSIM) were shown to work OK, but fail for montage 3D images.

How to extend VIF/MS-SSIM to 3 dimentionals?

The articles concerning these metrics and matlab codes implementing them can be found on Prof.Bovik site: http://live.ece.utexas.edu/research/quality/