Hey everyone! Have anyone faced an issue of how to minimize/quench autofluorescence in mammalian live cells? To be more specific I always stained and visualized ovarian cancer cell lines in blue, green, and red in confocal. And never witnessed any autofluorescence signal. But started working with ascites NO STAINING cells have so high intensive signal in any channel (blue, green, and red). Even at low laser power, there is a pretty obvious signal in all channels.
So maybe anyone had the same autofluorescence live cells issue? Or any ideas on how to quench it? Would really appreciate any advice!