I bought this cell line from ECACC as a frozen sample, and three weeks after thawing its growth is almost undetectable. I'm using the medium recipe recommended.
ZR-75-1 grows very fast. I think your cells altered in some aspects and you need to purchase a new one.
Thanks, even if I have read on the http://www.hpacultures.org.uk site that these cell line grows very slowly and does not go to confluency...any suggestions? Are you working with this cells at present?
We use RPMI or high glucose DMEM for ZR-75-1. Cell growth is also dependent of FBS, you have to check different suppliers or serum.
I bought the cells from ATCC about 6-8 months ago. they grow very slowly and it says so. Changing serum is an interesting thought.
I was working with this cell line for two years, I had no problems with them. They were grown in phenolred-free RPMI 1640 tissue culture medium (PAN Biotech, Aldenbach, Germany), supplemented with 10% fetal bovine serum (FBS) and 1% penicillin-streptomycin (Gibco Invitrogen). Did you check them for mycoplasma infection?
I am not sure what you mean by very slow. Mine grows slightly slower than MCF-7, but faster than Hs-578T or MDA-MB-468. If I split it 1:4, it takes about 5 days to become confluent. I do not change media, but rather feed them with extra media. I used Glutamax RPMI with 10% FCS and 1% Pen-Strep and they are mycoplasma free(tested)
Same as Debjani above I agree the ZR-75-1 cells have a slower doubling time than MCF-7 cells but they do grown up to confluency having being split 1:5. I also use the same media as above (with phenol) but the batch and supplier of FCS might dramatically effect growth rates.
Hi Alberto!
I have been working with ZR75.1 cells for more than 3 years now and they DO grow slow. In fact the paper that describes the establishment of this cell line states that the doubling time is ~80h (Establishment and Characterization of Three New Continuous Cell Lines Derived from Human Breast Carcinomas. Linda W. Engel,1 Nathaniel A. Young, Tommie Sue Tralka, Marc E. Lippman, Stephen J. O'Brien, and Mary Jo Joyce). In my hands that's the doubling time that I have by growing them in RPMI 1640 pen/strp, 5%CO2 and atmospheric O2.
But as suggested by other persons you may want to get another vial. These cells do not like to be too sparse so I'd recommend you to start with the new frozen stock by placing them in a T25 flask and then expand them as they grow. The do not reach confluency.
I agree with Denise. It takes about 80h when grown in RPMI-phenol red-free with 10% FBS. They are very different than the MDA-MB-453 which grow very quick even in conditions of low serum. It is tough calling ATCC fro another vial since they mentioned that that's how fast the cells grow.
I culture them in RPMI1640 with 10% FCS or BCS with 1 nM estradiol. (0.5 ml/ 500 ml medium), penicillin and streptomycin. These cells are estradiol dependent.
Hi,
Like everybody else, we find these cells growing very slowly. Some supplements certainly help, but we also find that keep the cells relatively dense keeps them happy. So, we split only 1:2 every 3-4 days. If splitting 1:5 it already takes at least one day to get them going again.
It seems everyone has their own recipe. I use RPMI, 10% FCS, 2mM Gln, and supplemented with non-essential amino acids. This works quite well.
I would like to know if you had success in growing the cells?
Also, do you use any kind of anti-microbial or anti-fungal additives in the medium? There is none listed in the medium supplementation and I am wondering if they could have an undesirable effect on the cells.
We stopped using this cell line and have turned our efforts to others, mainly MDA-MB-453 cells and MDA-MB-231. We usually add streptomycin to the media, but I do not think it has an effect on cell growth. Good luck
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