Any one familiar with SOFC/SOEC simulations using Ansys Fluent ?

23 August 2020 8,231 3 View

Adjust annealing temperature or additives to enhance specificity of PCR?

Dear All, I have a very basic question about the PCR/RT-PCR/qPCR. The PCR primers and TaqMan probes are designed such that the primers/probe connot bind to the mismatched site but only...

15 June 2020 4,926 3 View

Crystallization of PLLA?

07 June 2020 2,395 6 View

How to define grain boundary hardness?

08 March 2020 3,388 11 View

Will the TaqMan probe length affect the fidelity of the qPCR assay?

Dear all, I am learning to design the qPCR assays recently. But I just don't know why my negative controls always give positive signal( I have changed my design twice). I checked the formation of...

07 October 2019 4,968 3 View

Effect of mRNA secondary structure on reverse transcription?

Dear Sir/ Madam, I am designing a primer for microRNA reverse transcription. But some microRNAs like hsp-mir-21-5p has a strong secondary structure. It forms a stable hairpin. Can anyone please...

08 September 2019 8,283 3 View

How to deal with the nonlinearity in electrical tomography?

The electrical tomography can be utilized to calculate the solid concentration or gas holdup in aqueous based multiphase flows. However, there usually exists a strong nonlinear relationship...

16 May 2019 197 1 View

How many base-pairs can be added to the pSin vector?

Recently I decide to add a 5.5 kb fragment to pSin vector will it affects the efficiency of virus packaging?

20 March 2019 9,786 2 View

How can I deal with the non-specific binding in XIST-RNA-FISH?

I'm trying to detect the XIST-RNA expression in Breast cancer cells using the Biotin and Digoxin probes. However, there are always non-specific bindings in my results. I have added RNase-inhibitor...

18 March 2019 3,761 1 View

Any UV light absorbing material at 193-248 nm?

Would like to coat the alloy surface with non-organic material to absorb the UV light at 193-248 nm. Please recommend some UV absorbing material. Thanks~

16 November 2018 1,466 1 View

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02 March 2021 3,060 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

No title

28 February 2021 9,936 2 View