I am conducting callus induction using Murashige & Skoog (MS) and Murashige & Tucker (MT) media. I need information about all of the material and composition to make MT media. Can anyone recommend references?
MT Medium
MACROELEMENTS mg/L
NH4NO3 1650
KNO3 1900
CaCl 332.2
MgSO4 180.7
KH2PO4 170
Na-EDTA 37.26
FeSO4.7 H2O 27.8
MICROELEMENTS mg/L
H3BO3 6.2
MnSO4 . H2O 16.9
ZnSO4 . 7H2O 8.6
KI 0.83
NaMoO4 . 2H2O 0.25
CuSO4 . 5H2O 0.025
CoCl2 . 6H2O 0.025
ORGANICs mg/L
Vitamines
Myo-inositol 100
Thiamine - HCl 10
Pyridoxine-HCl 10
Nicotinic acid 5
Glycine 2
Sucrose 50.00
Malt extract 500
Agar 0.8%
pH 5.7
http://www.plantphysiol.org/content/62/6/885.full.pdf
http://hortsci.ashspublications.org/content/29/3/214.full.pdf
http://hos.ufl.edu/sites/default/files/courses/hos6373c/february%205/media2.pdf
http://www.jipb.net/pubsoft/content/2/2627/32-7-3.pdf
http://www.scielo.br/pdf/pab/v45n7/04.pdf
http://plant-tc.cfans.umn.edu/listserv/2001/log0111/msg00123.html
I am unable to get that exact paper..this informations may helpful to you..
Dear Dr. Raju Karthic,
Thank you very much for your quick response. Your information is very helpful for me.
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