How to modify Al2O3 surface to make it hydrophobic?

Dear Scientists, I am going to disperse Al2O3 with synthetic oil (hydrophobic). Therefore how to modify Al2O3's surface to it stable in the lubricant? thank you very much. Best regards, Agus.

25 August 2020 4,485 3 View

How to determine atom% from weight% as shown in Maud Crystallographic Software ?

My understanding is atom% can be obtained from weight% by dividing all latter values by the appropriate atomic weights and normalizing their sum to unity. However, I am having a difficulty in...

17 June 2020 5,309 5 View

How to preserve shrimp hemolymph?

Can anyone suggest how to preserve shrimp hemolymph sample during transport to laboratory from a remote shrimp farm? How to prevent hemolymph to clot between individual extractions in a pooled...

01 February 2018 5,287 1 View

Why DNA plasmid have to dry after wash by ethanol??

09 September 2017 7,373 4 View

Nitrification vs mineral concentration

Is nitrification directly affected by major minerals (Ca, Mg, K, Na) concentration in water? Does the nitrifying bacteria need these minerals significantly?

09 August 2017 2,095 3 View

Why should I use a reversed column for better peak in HPLC?

27 March 2015 8,813 3 View

How can I determine the stability of biosurfactant based on the emulsion activity test?

I have done a test of emulsion activity (and surface tension) to characterize the biosurfactant produced by bacteria. The emulsion activity reduced after 24 hours, but no more than 10%. How can I...

08 August 2014 9,155 9 View

Co-Immunoprecipitation problem - Can anyone lend their expertise?

I want to purify a protein complex with co-IP kit (Thermo-Pierce, resin column), but, at the end, I could neither elute enough specific protein nor visualise by WB using antibody against...

03 August 2014 6,377 6 View

Determination of Ginkgolide A and B using HPLC

Can anyone suggest a suitable solvent system in HPLC (using column of Licrhospher 100 RP-18 5um, Merck, Darmstadt, Germany) to determine Ginkgolide A and B ?

28 November 2012 4,169 18 View

Can you explain how to develop a good questionnaire?

A questionnaire is an instrument usually used in survey research. How to make a good questionnaire so that it can provide us the best results (valid, reliable) to our study?

12 September 2012 3,699 1 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View