Multiplexed PCR coupled with Flow cytometry?

07 February 2014 1 8K Report

Hi there, has anyone tested Luminex technology for BSI detection of pathogens? I'm having trouble finding validation studies (sensitivity, specificity against traditional microbiology).

Jennifer Gibbons

Your question is a little unclear. Since Luminex technology couples endpoint PCR with bead hybridization, the sensitivity is lower than qPCR. There are plenty of studies comparing qPCR and traditional microbiology- I suggest you look at those.

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