What is involved in morphological changes of SEM images of a substance?

In the sem analysis of CaCO3 nanaoparticles, the morphology images showed spherical shaped particles but the proposed morphology for these type of nanoparticles is trigonal. I prepared the...

01 March 2021 1,960 4 View

Want to ask about equivalence ratio?

09 February 2021 5,273 2 View

Which is an ideal solvent to dissolve CaCO3 nanoparticles?

I want to check antibacterial activity of CaCO3 nanoparticles, and I have to make suspension in which they can easily disperse. I have tried DMSO, chloroform, ethanol, saline and DI water, but...

04 November 2020 4,130 3 View

How To Keep A Network Running Even When The Server Is Down?

I was working on MIB server for educational purpose. But since a week I am unable to use the server. It is either restricted or down, I don't know what the problem is. But I want to dock my metal...

02 November 2020 8,775 3 View

What are the suitable conditions to probe sonicate CaCO3 nanoparticle dispersion before doing SEM measuremnts?

Need to know sonication time for SEM measurements of nanoparticles

07 September 2020 4,417 3 View

Sample size calculation mistake?

Dear friends I collected data in a questionnaire form with a total number of 500. I submitted my manuscript and now the reviewer asked me to verify sample size calculation. What will be the values...

31 August 2020 4,456 16 View

How to dissolve laurdan in DMSO?

08 March 2020 9,188 3 View

Topic for research project?

currently I am starting a educational research project, My area of interest is educational leadership and management and school improvement. In this regard i am searching for a burning topic. If...

25 August 2019 5,785 1 View

Regarding preprint, How can someone publish data as preprint before being submitted to journal?

Dear followers As journal instruct authors not to submit work that already been submitted elswhere, how can I publish preprint?

07 June 2018 5,202 2 View

How would the timing attack occur in fog computing? could anyone explain with an example?

fog computing ,side channel attack

14 March 2018 380 1 View

Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

What are some important techniques for protein induction and SDS-PAGE analysis?

Hello everyone. I am fairly new in protein chemistry field. I am trying to perform a protein induction hoping to purify my protein of interest using FPLC method. However, I am having trouble on...

03 March 2021 8,237 3 View

SDS page software for analysis?

I did sds page to determine the difference in protein expression between 3 types of vaccine , but i don't have scanner or densitometer available. . so i wonder if there is a software to analyze...

02 March 2021 6,270 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

How can I represent SDS-PAGE results for machine learning analysis?

I am trying to classify and analyze the results of an SDS-PAGE based array for bacterial detection using machine learning, but I have trouble finding the best way to represent the results with...

27 February 2021 9,176 3 View

Why tick's salivary gland extract didn't run in the gel (SDS PAGE )??

Greetings, I want to make SDS PAGE for my sample (Tick salivery gland extract), I tried concentrations 10%, 12%. I also tried high molecular weight ladder. But the protein stopped at the top and...

25 February 2021 7,698 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View

Quantifying leaking DNA from bacteria - what's the secret?

Hi, all! I need to determine if my treatment causes membrane damage in bacteria (S. aureus and E. cloacae), but I cannot use a fluorescent probe such as PI and similar ones because my compound is...

24 February 2021 2,006 2 View

Is there an ideal incubation time of RGO-modified SPCE before subsequent use, i.e., electrochemical characterization or further modification?

I notice that upon washing and air-drying of my RGO/SPCE surface and characterizing again via DPV, the current response towards ferricyanide probe significantly decreases. My worry is that the...

22 February 2021 5,324 3 View