My SHSY-5Y cell line morphology is changing on it's own and getting a morphology that resembles differentiated cell line. Could anyone provide me an insight of what is happening.
I am also attaching a picture of the cell line here with.
Possible causes:
1. Mycoplasma contamination - try to check whether there is mycoplasma by mycoplasma quick detection kit or mycoplasma culture
2. Too acidity - change the media before it turned completely yellow
3. Over trypsinization - never trypsinized more than 5 min
4. The culture medium never warm up to 37°C before and during culturing
5. Cell passage too late
1. Revive one vial from your stock and confirm the morphology. Probably you have cultured them for a longer time.
2. Mycoplasma contamination.
3. I hope you are not treating cells with any kind of selective drug which probably may sometimes affect the morphology.
The cells seem to be stressed by the looks of the image.
Were there any changes in the Co2 levels or are you observing any slight turbidity or anything indicative of contamination?
Check all the components again if they are not expired.
Hope it helps!
- Badges
- Science topic
- Similar topics
- Biological Science
- Molecular Cell Biology
- Culture Cells
- Cell Line