Rajsekhar Roy

5 Questions 5 Answers 0 Followers

Questions related from Rajsekhar Roy

Determination of specificity of amyloid oligomer towards NMDA receptor subtype?

What characteristic of amyloid beta oligomer decides its specificity towards the NMDA receptor subtypes like GluN2a and GluN2B?

28 April 2023 6,520 0 View

Rat hippocampal primary culture cell seeding?

We are trying to culture P-0 rat primary hippocampal culture. Could someone please advice me about the number of cells should be seeded in single well of a 6 well plate? Also, is there any...

25 June 2022 5,826 0 View

Morphology of SHSY-5Y is changing. Why?

My SHSY-5Y cell line morphology is changing on it's own and getting a morphology that resembles differentiated cell line. Could anyone provide me an insight of what is happening. I am also...

14 March 2022 7,473 3 View

What is the reason behind fluorescence in 4% PFA fixed tissue?

We are trying to perform IHC using fluorescence conjugated secondary antibodies for rat skin and brain tissue cryosections. What is the possibility of 4%PFA(in PBS pH=7.4) fixations to cause...

19 January 2022 8,411 3 View

What is the exact role of amyloid beta in glutamate mediated neuronal toxicity in neurons of hippocampus region?

Glutamate activates synaptic ionotropic NMDA receptors present in the post synaptic densities or PSDs, which sequentially activates the CAMK2 and CREB (cAMP-response element binding protein) that...

13 October 2021 7,365 5 View