Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

Is there any tutorial for comsol general projections?

Hi, im trying to make a projection in a 3d comsol model. I have a non uniforme chanel and my intention is to get the average tempreture in the y-z cross section and se the average tempreture...

02 March 2021 4,624 4 View

Expression cloning by using cDNA,do I need to modify the cDNA first?

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...

28 February 2021 5,440 3 View

I need dataset for recommandation system?

Hi every body I need to collect datasets from different source to work on recommendation system algorithms, could you advise me how collect data?

27 February 2021 7,063 7 View

How to estimate 2SLS with different instruments for different endogenous variables?

To be specific, I want to conduct an IV regression in stata with the following three equations. EQ1: Y1 ~Y2 Y3 X EQ2: Y2 ~ Z1 EQ3: Y3 ~ Z2 Both Y2 and Y3 are endogenous. However, Z1 and Z2 are...

23 February 2021 6,266 3 View

Which microtomy technique do you suggest for thin and delicate root sectioning ?And, how to preserve and process the roots before sectioning?

We have thin roots of from 1 year old different species of plants. We are looking into anatomical details of the roots. What's the best method to preserve and process the roots once they are...

23 February 2021 1,898 1 View

How to find out the problem of VMD software when i run it?

Hello I try to install and run the VMD software on windows 10 (64-bit).It doesn`t work and it is closed when I run it. I checked all of tutorials, videos and documents. But they did not work...

22 February 2021 8,211 3 View

Sample modeling in ABAQUS software and its relationship with the real world?

About crack modeling in ABAQUS: Mode I (pure-tensile cracking) and Mode II (pure-shear cracking), these assumptions may be accruing in theoretical approach (ABAQUS) not practical in Lab....

19 February 2021 6,156 1 View

Making my research papers available for readers?

17 February 2021 8,533 2 View

How does a freeze dryer destruct your solid sample?

Several times I lost my sample during freeze drying. There was no solid or powder in the vial after freeze drying. I'm sure sample contained solute because i took its NMR spectrum. I covered the...

15 February 2021 6,749 2 View

Which are the Non Small Cell Lung Cancer markers?

I would like to sort from a fresh specimen of lung cancer Non-Small Lung Cancer cells and cancer-associated fibroblasts to establish two different cell cultures. Which markers should I use? Thank...

04 January 2021 7,986 3 View

Which substance can I use as positive standard in MTT assay on A431 cell line (human epidermoid carcinoma)?

Hi everyone, I am planning to do an MTT assay for the cytotoxicity evaluation of several plant extracts on A431 cell line (human epidermoid carcinoma). For this I need to use a positive standard....

14 December 2020 1,470 4 View

Is there any possibility to buy specific drug resistant human and mouse tissue array from company or other research organisations?

I need some specific cancer tissue samples' slides for IHC which is resistant and sensitive to specific cancer drugs, especially for prostate, lung, and breast cancer. Is it possible to buy or...

27 November 2020 731 2 View

How can I make my Caki-2 cell line grow?

Hello everyone! I have trouble with a cell line, namely the human kidney carcinoma Caki-2 cell line. Another lab sent us a few criovials of cells, with their growing conditions, but I can't grow...

24 October 2020 2,568 3 View

Are my Small Cell Lung Cancer (SCLC) showing normal confluence and morphology?

Hello, I am new to cancer research and I am trying to optimize my small cell lung cancer cell protocol. I purchased a cell line from ATCC (H1105) and I have been growing the cells in RPMI 1640...

11 October 2020 5,670 6 View

What is the best mouse model for lung cancer study?

what is the best mouse model for lung cancer study? No Knockout Mice We Have Simple Strain?

27 September 2020 5,817 1 View

Should I perform transcardial perfusion by PBS before lung isolation for RNA expression analysis?

Hey, I am planning to dissect rats and isolate Lung tissue samples to be stored in RNA stabilizer for later analysis. - Should I perform trandcardial perfusion by PBS ? - Is it necessary to...

21 September 2020 1,822 5 View

I want to buy Human Squamous Cell Carcinoma Cell Lines for my phD research .?

i want to buy Human Squamous Cell Carcinoma Cell Lines for my PhD research .? One place is NCCS Pune...Can some give more suggestions..? Humble Regards

20 September 2020 3,940 3 View

Can the chemical compounds of the dragon fruit be a posible biomimicry cure for lung cancer?

My name is Erin and I am currently attending a biology class where we were discussing some posible cures for lung cancer that were based on biomimicry. After investigating, I found out that the...

02 May 2020 1,119 0 View

Which are the different immunity mechanisms our organisms possess in order to protect us from lung cancer?

I was wondering if our immunological system possessed any kind of immunological defense to prevent lung cancer and how it deferred (if any) from any other kind of immunological reaction.

30 March 2020 3,691 3 View