Moumita Sarkar

6 Questions 9 Answers 0 Followers

Questions related from Moumita Sarkar

No correlation between cell death and annexin staining?

I dump certain steroid treatments on my cancer cell lines followed by annexin staining. However even if i am observing under the microscope that there is significant cell death as all cells have...

09 June 2018 785 8 View

How to check whether my primer spans exon intron or exon exon junction?

I have been given some pre designed primers for performing qPCR and i have done the BLAST and have found that all the primers are specific to mRNA. However i would like to check that whether my...

06 September 2017 2,892 5 View

Why do i observe no correlation between Semiquantitative and Real time PCR ?

I am studying the expression pattern of few genes in tumor samples treated differently. Following cDNA making and PCR i can clearly see difference in gene expression on gel. However even though i...

01 September 2017 1,263 6 View

Morphology of Lewis Lung Carcinoma cells ?

ATCC describes the morphology of these cells to be round but when i grow these cells simply in the suggested complete media then i have often observed that the cells grow and appear like dendritic...

21 April 2017 2,020 1 View

Dna isolation from mouse blood?

i have been trying to isolate DNA from mice blood from a long time using different kits. Presently i am using QIAGEN kit to isolate DNA . Initially i was getting nothing on the gel but after...

29 January 2017 9,515 1 View

Ph of DEPC treated water?

I prepare DEPC water in Milli Q (0.1%) following the standard protocol. After leaving the preparation overnight i autoclave the solution as it is required for DEPC breakdown. But what i have...

17 January 2017 5,195 4 View