Moumita Sarkar

9 Questions 12 Answers 0 Followers

Questions related from Moumita Sarkar

A human cell line that can be a homologue for mouse LLC1?

I am using mouse lewis lung carcinoma cells as a model system in vivo for my studies. I am further interested to see the effects in a human lung cancer cell line that can be a close homologue of...

12 February 2020 497 0 View

Why there is a low count in proliferation assay of COLO-205 cells?

In my experiment i use tritium labeled thymidine for my cells and incubate for more than 24 hours before assessing the counts per minute as a read out for measuring proliferation of my cells....

25 June 2018 177 3 View

No correlation between cell death and annexin staining?

I dump certain steroid treatments on my cancer cell lines followed by annexin staining. However even if i am observing under the microscope that there is significant cell death as all cells have...

09 June 2018 847 8 View

Storage temparature of cell supernatant containing steroids?

I want to store my cell supernatant for further use in assays for detection of steroid in it. Hence what is the optimal temparature in this case for storage of supernatants so that the steroids...

12 September 2017 1,188 3 View

How to check whether my primer spans exon intron or exon exon junction?

I have been given some pre designed primers for performing qPCR and i have done the BLAST and have found that all the primers are specific to mRNA. However i would like to check that whether my...

06 September 2017 2,940 5 View

Why do i observe no correlation between Semiquantitative and Real time PCR ?

I am studying the expression pattern of few genes in tumor samples treated differently. Following cDNA making and PCR i can clearly see difference in gene expression on gel. However even though i...

01 September 2017 1,323 6 View

Morphology of Lewis Lung Carcinoma cells ?

ATCC describes the morphology of these cells to be round but when i grow these cells simply in the suggested complete media then i have often observed that the cells grow and appear like dendritic...

21 April 2017 2,066 1 View

Dna isolation from mouse blood?

i have been trying to isolate DNA from mice blood from a long time using different kits. Presently i am using QIAGEN kit to isolate DNA . Initially i was getting nothing on the gel but after...

29 January 2017 9,557 1 View

Ph of DEPC treated water?

I prepare DEPC water in Milli Q (0.1%) following the standard protocol. After leaving the preparation overnight i autoclave the solution as it is required for DEPC breakdown. But what i have...

17 January 2017 5,285 4 View