6 Questions 9 Answers 0 Followers
Questions related from Moumita Sarkar
I dump certain steroid treatments on my cancer cell lines followed by annexin staining. However even if i am observing under the microscope that there is significant cell death as all cells have...
09 June 2018 783 8 View
I have been given some pre designed primers for performing qPCR and i have done the BLAST and have found that all the primers are specific to mRNA. However i would like to check that whether my...
06 September 2017 2,889 5 View
I am studying the expression pattern of few genes in tumor samples treated differently. Following cDNA making and PCR i can clearly see difference in gene expression on gel. However even though i...
01 September 2017 1,261 6 View
ATCC describes the morphology of these cells to be round but when i grow these cells simply in the suggested complete media then i have often observed that the cells grow and appear like dendritic...
21 April 2017 2,017 1 View
i have been trying to isolate DNA from mice blood from a long time using different kits. Presently i am using QIAGEN kit to isolate DNA . Initially i was getting nothing on the gel but after...
29 January 2017 9,513 1 View
I prepare DEPC water in Milli Q (0.1%) following the standard protocol. After leaving the preparation overnight i autoclave the solution as it is required for DEPC breakdown. But what i have...
17 January 2017 5,192 4 View