Dear colleagues,
Can I culture rat cortical cells on supplemented DMEM-F12? Is there a paper comparing medium culture conditions?
Sincerely,
Hércules Freitas
Please check the method seciton in article "
Freshly frozen E18 rat cortical cells can generate functional neural networks after standard cryopreservation and thawing procedures"
The authors use supplemented DMEM-F12 in primary culture of rat cortical cells .
Hi, all! I need to determine if my treatment causes membrane damage in bacteria (S. aureus and E. cloacae), but I cannot use a fluorescent probe such as PI and similar ones because my compound is...
24 February 2021 2,006 2 View
Hi. I'm using the PyRx version 0.8 and getting the error message: WARNING: Filename mismatch: "2DFN_ADP_CL.maps.fld" :: "..\2DFN_ADP_CL.maps.fld" C:\Program Files (x86)\The Scripps Research...
17 January 2021 843 3 View
I have altered two instruments and collected a sample of n=60. 1. For the first instrument, I changed the wording of the items to refer to life instead of work. I believe checking the cronbach...
13 March 2020 7,391 3 View
Hi all, I have high levels of protein loss on PD-10 desalting column (gavity flow desalting column) when trying to buffer exchange. According to GE, the recovery rate should be higher that 70%,...
03 October 2019 8,483 3 View
I am new in gromacs and I'm experiencing some difficulties in the analysis of my results. I calculated the RMSD of backbone and ligand from the MD with a trajectory of 100ns (nsteps = 50000000),...
04 September 2019 3,669 6 View
Hi I'm trying to simulate the flow of water over an ogee spillway in Fluent. My problem is that I'm getting reversed flow on my pressure outlets. I am aware that it might not affect significantly...
05 December 2018 6,108 3 View
I've isolated exosomes from 5ml of cell culture media using ExoQuick TC PLUS kit. I got a visible pellet that was resuspended in the "resuspension buffer" and then purified with the...
13 June 2018 5,514 4 View
I am trying to decide the best method between flow cytometry or qPCR to be used as I compare Glut1 expression in two different T cell lines. Is there an ideal protocol? I had read articles where...
17 March 2018 4,211 3 View
Hello, I am beginner in Materials studio software and also in Molecular dynamics and I am needing to build a silica nanosphere with different numbers of hydroxyl groups to adsorb some molecules....
27 December 2017 5,577 2 View
15 November 2017 8,055 2 View
Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...
24 February 2021 1,825 3 View
Hi, I am currently working on a pilot study to get some data related to the impact of long duration spaceflight to the microbiome and the immune system. I plan to use rats and simulate...
22 February 2021 592 1 View
I am working on C6 cells along with other neuronal cell lines. We have preserved the cells in 90%FBS and 10% DMSO solution. We are reviving the cells after a year and cells are either not binding...
18 February 2021 4,499 1 View
Hello everybody In our iPSC-derived neuronal culture we use Maturationmedium, supplemented with B27+. As Thermo Fisher run out of stock and can deliver earliest in May, I wanted to ask whether...
16 February 2021 3,348 1 View
I'm working on organotypic cerebellar slice cultures from postnatal rats and I'm not sure how to tell if the slices are healthy at any given point. I know there should be a lot of microglial...
15 February 2021 2,830 2 View
rats were given daily dexamethasone in drinking water corresponding to 0.5 mg/kg or 2 mg/kg. These rats are simultaneously fed 25% fat, 3% salt in diet and 10% fructose in drinking water but...
15 February 2021 7,634 2 View
We are trying to inject insulin to rat or mouse, but the insulin we've got is powder, and there are many ways for dissolve, like in saline, PBS, DMSO, or in Tris-HCl, can anyone give us a...
13 February 2021 8,426 10 View
I am cutting rat brain sections at 20um at -20C. The tissue is cutting nicely out of the O.C.T solution, but I can not get a decent section to adhere to the slide. I have included an image.
09 February 2021 1,345 5 View
Hello everyone, Has anyone try to culture the normal prostate RWPE-1 cell line in RPMI medium supplemented with foetal bovine serum? There's some articles available but neither describe details...
08 February 2021 6,455 1 View
Hello, I'm trying to identify good putative macrophage markers for flow cytometry in rats.
04 February 2021 3,913 4 View