Where to find a specific lipid inhibitors?

Dear all, I need to find a chemical lipid inhibitor for a specific pathway. Where can I find the information about these inhibitors? Thank very much in advance julien

11 December 2017 9,812 2 View

Any suggestion for an RNA extraction robot?

Dear all, I am planning to buy a robot to extract RNA. Any suggestions on which one is the best? Thanks

01 February 2017 2,632 5 View

Is anyone familiar with double Indirect ELISA?

Merry christmas all, I want to do an indirect ELISA and detect 2 proteins at once in the same well. To do this end, I would like to use two secondary antibodies with different fluorophores, like...

11 December 2014 3,967 5 View

What humidifier should you buy to regulate humidity in an insectary?

Hello, I just arrived in a new lab with an insectary that has no built-in humidity regulator. I am looking to purchase a humidifier that can regulate humidity, therefore one that can at the same...

09 October 2014 280 4 View

Is anybody familiar with SILAC Schneider medium?

Hello, Do anyone know where I can find SILAC Schneider medium? If does not exist, is there an easy to make it myself, like adding the isotope only in medium just lacking Lysin and Arginin? Thanks

08 September 2014 3,101 4 View

How to establish cell lines in insects?

I am interested in establishing new cell lines in insects. Do you have any advice with regards to the protocol? Thank you very much.

31 December 2013 2,359 3 View

Can someone answer a question regarding PenalizedLDA?

I am trying to select a subset of variables from a dataset containing more features (p = 100) than observations (n = 18) by using PenalizedLDA. I changed all value in numeric values for the...

05 June 2013 907 2 View

How to compute a backward stepwise discriminant analysis with R?

R is a great statistic language but sometimes it is hard to navigate through the different algorithms and find your way when you're not a professional statistician like I am. I would like to...

03 April 2013 2,339 13 View

How to deal with multicolinearity?

I have a proteomic dataset with more than a hundred proteins in different conditions. I would like to run a stepwise discriminant analysis to select a subgroup that is discriminating among my...

03 April 2013 2,826 9 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

No title

02 March 2021 3,060 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Do you know any MR image dataset?

I would like to research on MR images (0.5T and 3T). Can you please suggest some websites that I can download dataset including both 0.5T and 3T MR images? Thank you.

02 March 2021 7,735 3 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

What are the countries most affected by extreme rainfall as a result of climate change?

Increased heat, drought and insect outbreaks, all associated to climate change, have increased wildfires. Decreasing water supplies, declined agricultural yields, health impacts in cities owing to...

01 March 2021 7,168 9 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View