Where to find a specific lipid inhibitors?

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Any suggestion for an RNA extraction robot?

Dear all, I am planning to buy a robot to extract RNA. Any suggestions on which one is the best? Thanks

01 February 2017 2,767 5 View

Mitogen / growth factor for insect cells?

Dear all, We are trying to generate primary cell lines from mosquitoes but strugggle in having them dividing. Anyone know a growth factor of insect cells from Drosophila for instance. Thank you

06 July 2016 4,150 1 View

Is anyone familiar with double Indirect ELISA?

Merry christmas all, I want to do an indirect ELISA and detect 2 proteins at once in the same well. To do this end, I would like to use two secondary antibodies with different fluorophores, like...

11 December 2014 4,096 5 View

What humidifier should you buy to regulate humidity in an insectary?

Hello, I just arrived in a new lab with an insectary that has no built-in humidity regulator. I am looking to purchase a humidifier that can regulate humidity, therefore one that can at the same...

09 October 2014 473 4 View

Is anybody familiar with SILAC Schneider medium?

Hello, Do anyone know where I can find SILAC Schneider medium? If does not exist, is there an easy to make it myself, like adding the isotope only in medium just lacking Lysin and Arginin? Thanks

08 September 2014 3,251 4 View

How to establish cell lines in insects?

I am interested in establishing new cell lines in insects. Do you have any advice with regards to the protocol? Thank you very much.

31 December 2013 2,499 3 View

Can someone answer a question regarding PenalizedLDA?

I am trying to select a subset of variables from a dataset containing more features (p = 100) than observations (n = 18) by using PenalizedLDA. I changed all value in numeric values for the...

05 June 2013 1,038 2 View

How to compute a backward stepwise discriminant analysis with R?

R is a great statistic language but sometimes it is hard to navigate through the different algorithms and find your way when you're not a professional statistician like I am. I would like to...

03 April 2013 2,494 13 View

How to deal with multicolinearity?

I have a proteomic dataset with more than a hundred proteins in different conditions. I would like to run a stepwise discriminant analysis to select a subgroup that is discriminating among my...

03 April 2013 2,965 9 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View

Can i use the protease inhibitors during cell membrane vesicle preparation ？?

I am currently working on a project that involves extracting cell membranes, for which we disrupt the cells using sonication. During the initial extraction process, we add protease inhibitors to...

30 July 2024 7,077 1 View

Optimal siRNA Volume for Complexation with Liposomes in a 2.5 mL Solution with 10 mg/mL Lipid Concentration?

How much volume of siRNA can I add to 2.5 mL of liposomes with a total lipid concentration of 10 mg/mL?

27 July 2024 6,920 1 View

How to dispose off lipids waste?

I am looking for a lipid waste disposal method, keeping in mind the environmental, health and safety aspect of lipid waste. Could someone please provide guidelines or the lowest acceptable...

25 July 2024 637 5 View

How to select relevant metabolites from multiple annotations ?

I am using the Polyomics Integrated Metabolomics Pipeline (PiMP) for metabolite feature analysis. After annotating my data, I have encountered cases where multiple potential metabolites are...

24 July 2024 5,789 0 View

Optimal siRNA Volume for Complexation with Liposomes in a 2.5 mL Solution with 5 mg/mL Lipid Concentration?

How much volume of siRNA can I add to 2.5 mL of liposomes with a total lipid concentration of 5 mg/mL?

18 July 2024 9,831 0 View

How to prepare bacterial conditioned media to study the effect of bacterial secretome?

I would like to investigate the effect of bacterial secretome on cancer cells. How do you prepare conditioned media from bacteria?? conditions- time points - media I am looking for the best way...

17 July 2024 3,785 9 View

What does an additional peak in FTIR between 3100 and 3300 nm affect the quality of the material, benzethonium chloride USP??

I do not know much about FTIR! The material Benzethonium Chloride USP was compared to a reference standard in regards to FTIR, as per the USP testing: Identification B. Infrared absorption (FTIR),...

16 July 2024 6,918 7 View

How do you determine the Ki of an inhibitor for an enzyme which also undergoes substrate inhibition?

There are three factors involved in the activity, the substrate acting as a substrate, the substrate acting as an inhibitor and another inhibitor for which you want to measure the Ki.

08 July 2024 4,988 1 View

How is the preparation to obtain secondary metabolic extracts?

Hi everyone! I am working with bacteria and fungal collected from marine sediments. I would to know the methodology since the preparation of biomass until the extraction and stored of secondary...

03 July 2024 6,177 3 View