Where to find a specific lipid inhibitors?

More Julien Pompon's questions See All

Any suggestion for an RNA extraction robot?

Dear all, I am planning to buy a robot to extract RNA. Any suggestions on which one is the best? Thanks

01 February 2017 2,632 5 View

Mitogen / growth factor for insect cells?

Dear all, We are trying to generate primary cell lines from mosquitoes but strugggle in having them dividing. Anyone know a growth factor of insect cells from Drosophila for instance. Thank you

06 July 2016 4,010 1 View

Is anyone familiar with double Indirect ELISA?

Merry christmas all, I want to do an indirect ELISA and detect 2 proteins at once in the same well. To do this end, I would like to use two secondary antibodies with different fluorophores, like...

11 December 2014 3,967 5 View

What humidifier should you buy to regulate humidity in an insectary?

Hello, I just arrived in a new lab with an insectary that has no built-in humidity regulator. I am looking to purchase a humidifier that can regulate humidity, therefore one that can at the same...

09 October 2014 280 4 View

Is anybody familiar with SILAC Schneider medium?

Hello, Do anyone know where I can find SILAC Schneider medium? If does not exist, is there an easy to make it myself, like adding the isotope only in medium just lacking Lysin and Arginin? Thanks

08 September 2014 3,101 4 View

How to establish cell lines in insects?

I am interested in establishing new cell lines in insects. Do you have any advice with regards to the protocol? Thank you very much.

31 December 2013 2,359 3 View

Can someone answer a question regarding PenalizedLDA?

I am trying to select a subset of variables from a dataset containing more features (p = 100) than observations (n = 18) by using PenalizedLDA. I changed all value in numeric values for the...

05 June 2013 907 2 View

How to compute a backward stepwise discriminant analysis with R?

R is a great statistic language but sometimes it is hard to navigate through the different algorithms and find your way when you're not a professional statistician like I am. I would like to...

03 April 2013 2,339 13 View

How to deal with multicolinearity?

I have a proteomic dataset with more than a hundred proteins in different conditions. I would like to run a stepwise discriminant analysis to select a subgroup that is discriminating among my...

03 April 2013 2,826 9 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

What element is driving the different slop in the low frequency tail?

My study consists simple corrosion inhibitors in salty brine. Right now I just fit my circuit with randles with CPE. Just wondering how the slop can given more info of the experiment. Or any...

01 March 2021 6,474 3 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

Which database would give me this list of chemical components?

Dear colleagues: I would like to know if anyone knows of a database that gives me the parameters pH, K, K, Mg, P, C, N, Zn, Cu, Ni and Cd of common foods. I find a lot of information of proteins,...

26 February 2021 5,525 3 View

Can I use a protein-devoid condition as my negative control in ELISA?

Hello, I am running a sandwich-type ELISA where I am trying to inhibit a protein-protein interaction using small molecules. For each experiment, I determine my Z' (Z-prime) to determine the...

23 February 2021 7,429 1 View

How to calculate the fraction metabolized of a drug by an enzyme using clearance(ml/min) values of the metabolite formed?

I am trying to find the fraction metabolized of a drug by CYP and UGT enzymes. I am unable to get the values of fraction metabolized (Fm) directly from the literature. Can we calculate the Fm of a...

21 February 2021 5,379 2 View

Did anyone perform CD107a degranulation assay without the use of inhibitors like monensin?

I am planning to perform a degranulation assay using CD107a antibody. Some resources suggest to use inhibitors like monensin to inhibit the intracellular traficking and acidification of the...

21 February 2021 6,260 3 View

How to visualise pathways from a phosphoproteomics dataset?

I am looking for something similar than Ingenuity Pathway Analysis (IPA) in which I can enter the proteins differentially phosphorylated and I can visualise the pathways these proteins are...

21 February 2021 8,923 4 View

Phenol-pH graphic?

We want to use phenol pH graphic to measure the pH of mediums before and after inhibitor application to cells. We have phenol absorbence values at 460, 560 and 720 nm wavelength. When we measure...

20 February 2021 7,700 3 View