Mitochondrial DNA and whole mitochondrial genome sequencing?

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Reason (s) for retraction of articles?

Recently, through social media i came across several issues of article retraction. Where, blots were even duplicated, FACS results were duplicated, blots were manipulated and so on.... What are...

02 March 2018 7,715 4 View

Microarray Data Analysis?

Hello, Which software can be used (free) for the analysis of micro-array data other than GESA and GenePattern ? Best Regards

01 February 2018 4,073 3 View

HEK293 and HEK293T cell lines?

What is the difference between HEK293 and HEK293T cells? HEK293T is also written as 293T cells? Thanks Regards

11 December 2017 3,268 1 View

Complex I activity determination of mitochondria?

Hello, What method can be used for the determination of complex I activity of mitochondria (cultured cells/fresh tissue)? Thanks Best Regards

11 December 2017 7,703 4 View

Principle Component Analysis (PCA) Analysis?

Which software can be used for PCA analysis? where i can draw the graph and interpretate it? Any guideline how PCA analysis can be done using SPSS... Regards

10 November 2017 6,501 20 View

Difference between the SCI (Science Citation Index) Journal and SCIE (Science Citation Index Expanded)?

What is the basic difference between between the SCI (Science Citation Index) Journal and SCIE (Science Citation Index Expanded)? Does it matters in case of quality of journal? Thanks Regards

10 November 2017 1,460 9 View

Criteria for the distribution of Impact Factor between author(s)?

What is the criteria of IF distribution between the authors? It varies from institution to institution or there is any fix rule? If the IF of journal is 5.0 how it will be distributed between...

10 November 2017 2,500 4 View

Paper should be written before/after thesis?

Hello, What is advisable for a PhD student to write thesis first or publish first? or both can be done simultaneously? Best Regards

10 November 2017 9,329 4 View

HEK-293T can be used as normal cell line?

HEK-293T cell line can be used as normal cell line if i am investigating the anticancer potential of a compound against a panel of cancerous cell line (considering HEK-293T as normal among the...

10 November 2017 4,416 3 View

Opportunity of writing a chapter for book?

Hello, How a beginner researcher can find the opportunity of writing a chapter for book in his/her field of interest? Thanks Best Regards

10 November 2017 7,273 2 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View