Do you have some experience in isolation of miRNA from frozen plasma (-80) after a long period of time (about 12-18 months)? Are there some stabilizers to improve quality of samples? Could you share with me your experiences. What is the key to gather samples of good quality?
can anybody send the protocol for isolation of micro RNA from blood serum?
This article may help you. They analyzed the feasibility of using circulating microRNAs from plasma after a long period of time.
http://www.ncbi.nlm.nih.gov/pubmed/23499963
Hi Dawid
At NXT-Dx we offer miRNA expression profiling solutions (qPCR, array or sequencing) and we do have experience with samples that have been frozen for longer periods of time. We have actually seen that this worked well with our qPCR technique. There was a bit of degradation but not to such an amount that the samples couldn't be run anymore.
At NXT-Dx, we offer miRNA expression profiling using the latest in qPCR technology. We have compared qPCR,with arrays and sequencing techniques, especially in body fluids such as serum. The qPCR approach clearly comes forward as the optimal solution. On top of this we specialize in low-input approaches with this technique, so are very capable in delivering high quality data from serum samples. We can also take care of the RNA isolation from your serum samples before starting the
If you are still planning to run this experiment and would be interested in learning more about our approach, please send me an email on [email protected].
Maarten Braspenning
NXT-Dx
- Badges
- Science topic
- Similar topics
- Biological Science
- Biochemistry
- Biomolecules
- Nucleic Acids
- RNA
- Small RNA
- microRNA