I see a new term on RG statistics page "Total Research Interest":What does this interpret?

"Total Research Interest" is some new index. Can someone explain what does this imply? Maybe its a simple entity but iam not aware of this. Regards

01 February 2019 2,837 26 View

No title

31 December 2018 1,881 30 View

Should basic medical college training other allied medical specialties incorporate managerial sciences and information technologies?

The world is changing fast and lot many biotechnologies are now being routinely utilized by medical professionals in all domains. However, they do not have a formal training in this regard....

10 November 2018 2,917 24 View

How do you compare glucometer readings with lab gkucose results?

While both glcometer & lab glucose measuring technologies use different methodologies and sample. Still from physician to medic compare their results. What do you trust more & for what...

10 November 2018 5,017 7 View

I was looking at the publication fee before depositing an article for review. Can anyone explain what these terms mean?

Dear ALL Creative Commons Attribution (CC BY) Creative Commons Attribution-Non Commercial-No Derivs (CC BY-NC-ND) I am not sure what these publication options means.Obviously i intend to pay...

10 November 2018 1,860 16 View

Do you think genomic medicine will replace conventional medicine ?

Genomic data is emerging fast and every something new molecular research data is there. So what is you take on my question?

10 November 2018 7,222 23 View

Should we as healthcare worker waer our white coats/hospital dress outside the hospital?

I believe we are spreading more infection and antibiotic resistance if we are taking/wearing our white coats (the trade mark of ant medic) outside hospital. Should we do that? Are we not spreading...

09 October 2018 3,555 15 View

What are the differences between miRNA,siRNA, piwiRNA and lncRNA? ?

I will appreciate if u give specific differences between siRNA and miRNA and other types of RNAs. If anybody could elaborate. Regards Dr Sikandar

08 September 2018 5,545 2 View

If you are writing a mini review can you add a scematic from somebody's article after referencing?

This question is about writing a mini review where i want to add a schematic from some article after appropriate referencing.Can i do that?

08 September 2018 5,590 4 View

What is the preferenable bioniformatics base for RNA base alignments?

Google search and different RNASeq experiments highlights many types of RNA bases for read alignments. Can someone suggest a user friendly base fro small micro RNA alignments to known sequences?

07 August 2018 3,011 2 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Can MicroRNA cross the blood brain barrier?

In diseased conditions, many genes and gene products are visible in the bloodstream. So my question is whether we can find traces of microRNA which is involved in disease and disorder in blood serum?

24 February 2021 7,064 3 View

Are exogenous molecules transferred between human cells during doubling ?

Hello RG community, I wonderer whether exogenous molecules (e.g. reporter plasmids, miRNAs or other artificial molecules) are transferred between cultured human cells during their doubling ? So,...

21 February 2021 6,633 3 View

Has anyone encountered any problems with abm good microRNA primers for quantification of mature miRNAs?

I've been trying to quantitate mature microRNAs in 293T cells with and without 'X'. 2ug of total RNA (isolated by Trizol method) is converted to cDNA using All-in one microRNA cDNA synthesis kit...

17 February 2021 8,986 2 View

What is the best way to fix plasma derived miRNA for subsequent cDNA synthesis?

I need to quantify blood plasma derived miRNA through q-RT-PCR. For this I need to fix RNA content in my cDNA reaction. What is the best way to do this? Currently, I take readings of my plasma...

03 February 2021 9,330 3 View

What is the general miRNA yield one gets from the Qiagen miRNEASY serum/plasma advanced kit?

I am using the Qiagen miRNEASY serum/plasma advanced kit, using 400ul of frozen plasma. What is the yield you get on average? I am using nanodrop currently for quantification. I am getting...

02 February 2021 8,806 2 View

Which miRNA arm is a right target for analysis, 3p or 5p?

I'm working on selecting mouse miRNAs to analyze and stumbling upon one confusing issue, some of the papers do not specify which arm they analyzed and they just state something like " miR-34a was...

16 January 2021 3,792 4 View

Is negative controls necessary for western blotting?

I am trying to confirm my miRNA target by western blotting. I have a ds mimic and a ss inhibitor of my miRNA. Is it necessary to use negative controls for mimic and inhibitor? If yes, how can I...

09 January 2021 2,320 3 View

Can I predict miRNA by knowing the mRNA target?

Hello! I am trying to predict in silico the miRNA by knowing the target. Most of published research starts by knowing the miRNA and predicts the target using online tools. Are you familiar with...

31 December 2020 7,433 5 View

What are the possible drawbacks of using miRNA sponge to enhance the specificity of gene expression?

I am searching for miRNA repeats put between WPRE and 3'LTR of my lentivirus, in order to enhance the specificity of transgene expression in human neuron cells. But if clinically translation of...

26 December 2020 9,921 2 View