what is the simplest ways to decontaminate an infected incubator and laminar from fungal contamination ?
10% bleach from department store - wipe all surfaces and let dry
70% ethanol - wipe all surfaces and let dry
Arseniy Yuzhalin is that method effective against persistent contamination ?
Spraying or fumigation might be needed to reach all (contaminated) spots.
We fumigated our poultry houses with formaldehyde flakes. This requires heating and good containment of the fumigant for about 24 hours. A description can certainly be found in the practical advises for the poultry industry.
Make sure beforehand that the fungus or fungi are sensible to this treatment. Otherwise your effort will be useless.
Use formaldehyde for 24 hours. Then later wipe the inside surfaces with 70% ethanol.
Culture the fungus on e.g. a plate and expose it to different treatments to ensure its sensitivity just like you test for antimicrobial sensitivity of microorganisms.
C.A. (Kees) Kan oh , that means that contamination may be impossible to be eliminated ? and which spray / sprays do you recommend ?
PFA = Paraformaldehyde See above. I would use all three in succession. Start with PFA, then Bleach, then EtOH. You may also want to consider Hydrogen peroxide instead of Bleach.
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