what is the best protocol to culture lymph node from fresh tissue sample ? best media , best serum concentration , any other additives to be added ?
Depends on which cells you want to grow. I've grown lymph node B cells before in the AIM-V media (Thermo fisher). If you wish to grow endothelial cells, blood or lymphatic, you can use the endothelial cell growth median MV2 (Promocell). If you wish to grow fibroblasts you can use fibroblast medium (Sciencell). All growth supplements are optimised in these media ready to go right off the bat. But I don't know a medium that was suitable for all lymph node cells
Tommy Lu thank you , and there is a growth factor or induction in case the cancer is low grade ? and your method is suitable to produce stable cell line ?
Amr Mahmoud RPMI is generally used for culturing lymphocytes, I've grown primary T cells and B cell lines in RPMI and they are fine. I have not used these media for generating cell lines so I can't comment on which is more suitable for your procedure. AIM-V might be a good shot for lymphocytes if you wish to avoid bovine serum supplementation. Good luck.
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