I have mostly been using the immortalised C2C12 murine cell line for in vitro skeletal muscle atrophy studies. In my lab, I used serum-free DMEM for 24 hours to stimulate muscle atrophy in C2C12 myotubes, which was validated via upregulation of Atrogin-1 and MurF1 through RT-qPCR. I'm planning on using the human immortalised myoblast cell line LHCN-M2 for validation. Reading the protocol I found serum-free media is required for differentiation. So my question is what methods can I use to mimic atrophy in LHCN-M2 myotubes?
I know that there are plenty of papers on corticosteroid-induced atrophy in vitro, though I'm not familiar with what the appropriate dosages would be for LHCN-M2 myotubes.
Look up Dexamethasone... it is a very common drug used to induced atrophy in vitro!
Muscle cell cultures are already in an atrophy state, i.e., there is minimal (if any) contractile loading. Perhaps it would be better to go in the other direction, i.e., increase contractile loading, so as to promote "hypertrophy". Electrically stimulate the cell cultures. This is harder than it sounds. Too much stimulation can kill cells.
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