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Questions related from Joseph Hoolachan
For an assay I am planning to run I require a glucose-free MEM-alpha medium, I've seen papers where researchers state they use glucose-free MEM-alpha but they do not provide a supplier or catalog...
03 May 2024 3,980 0 View
Hi, I'm just enquiring what seeding density people use for LHCN-M2 myoblasts and myotubes? Also how long do they leave RNAiMAX in the media for transfection before replacing with fresh media?
20 February 2024 2,603 0 View
I'm investigating a novel target that is present in both cell surface plasma membrane and outer mitochondrial membrane. In skeletal muscle cell line I am running mitochondrial fractionation as I...
13 October 2023 1,567 0 View
I am working with the human LHCN-M2 myoblast cell line which I culture on 0.1% gelatin coated plates. For the myoblast stage this cell line works fine for attachment and growth. However, when I...
19 December 2022 1,607 3 View
A major part of my lab investigations is running radioactive 2-DG assays to measure glucose uptake of L6.GLUT4myc myotubes under various conditions. For the insulin stimulation at 100 nM, I have...
08 November 2022 1,881 0 View
My new lab is interested in using the human immortalized LHCN-M2 muscle cell line as an in vitro model in our research. I've been exploring the internet and reading the protocols. However, one...
04 March 2022 3,205 0 View
I've began working with the immortalised human myoblast cell line LHCN-M2. I've done a differentiation protocol and morphologically under the microscope its worked really well. I tried to run an...
26 April 2021 7,550 7 View
We are administering mifepristone as a therapy for pup mice up to 21 days old, unfortunately due to that age normal vehicles this drug can dissolve in like EtOH and DMSO were found to be toxic. We...
24 November 2020 8,729 0 View
I have mostly been using the immortalised C2C12 murine cell line for in vitro skeletal muscle atrophy studies. In my lab, I used serum-free DMEM for 24 hours to stimulate muscle atrophy in C2C12...
17 November 2020 502 2 View
I'm trying to model muscle atrophy in vitro in C2C12 cells that have been differentiated for 7 days by high glucose DMEM with 2% HS and 1% Pen/Strep. I was just wondering if I use high glucose...
03 March 2020 152 9 View
I am working on drug testing in C2C12 cells at myoblast and day 7 differentiated myotube stages. My drug of choice needs to be dissolved in DMSO. I have a stock solution at 8mM and to obtain a 100...
28 February 2020 2,725 3 View
