Hello community,
I am kindly asking for your help.
I was wondering if someone knows/or has used a protocol for extraction of amino acids and antioxidant amino acids from microbial culture (bacterial and yeast).
I want to quantify the amino acids in GC.
Thank you so much for your kind help.
I am truly grateful
Michael Kohlstedt
Dear Teresa,
most convenient is a fast filtration of cell broth followed by a washing step and metabolite extraction in boiling water.
Find the protocols here:
https://pubmed.ncbi.nlm.nih.gov/17411014/
https://pubmed.ncbi.nlm.nih.gov/18604477/
Obtained extracts need to be dried and the contained amino acids have to be derivatized with MBDSTFA to become measurable in GC.
Good luck
Michael
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