What are the antimicrobial categories and agents that are currently considered to define a MDR, XDR and PDR Salmonella?

What antimicrobial categories and agents are currently considered to define non-typhoid Salmonella as multidrug resistant (MDR), extremely / extensively drug resistant (XDR) or pandrug resistant...

19 November 2020 755 3 View

What are your dreams for ICM?

Congratulations & Good Wishes ICM Celebrates 3rd Anniversary If there is ever a corporate history written on ICM, it will start with you and end with you. You are not just another amazing...

24 July 2020 6,454 3 View

Where to purchase Ba(MnO4)2 for Hollandite synthesis?

Dear all, for synthesis of Ba-Hollandite (BaMn8O16) described in: Cheng, S., Lin, J. T., Ocelli, M. L., & Robson, H. E. (1992). Expanded Clays and Microporous Solids (p.335 & 337), I need...

30 June 2020 3,846 7 View

Can anyone please explain about the significance of powder XRD in characterizing phosphor materials?

Greetings everyone, hope you are all doing well. I just want a clear definition about how we can analyze the structural morphology of RE doped phosphors with powder XRD and what are all the...

12 May 2020 6,567 4 View

What is the best way to do immunohistochemistry on perfused gut tissue from rat?

We have recently collected perfused whole mount sections of the rat gut, containing the duodenum, ilium and colon. Following perfusion the sections were cut longitudinally but kept all in one...

02 April 2020 328 3 View

Is it possible that in the future the softwares of any spectroscopy system can run on a dedicated OS on dedicated computers?

I'm asking this question because current modern Spectroscopy machines, which are fully based on digital electronics can become obsolete or their upgrading is extremely expensive everytime there...

11 November 2019 6,531 3 View

I need expert feedback. Can someone test my questionnaire?

Hello everybody, I need feedback on my survey before I publish it. I would be happy if someone could test it first. The survey is (unfortunately) in German. It is about competencies of founders in...

26 July 2019 5,288 3 View

What is the duration of pollinator activity after they emerge?

I have been looking for information about the duration of the activity of pollinators after they emerge. I am aware that this will vary among species. However, there may be some average value. I...

06 May 2019 6,458 4 View

How to synthesize pure feitknechtite?

04 May 2019 5,432 1 View

Does anyone have a QPCR assay for SV40 Large T antigen?

I would like to do QPCR for metastasis for a cancer model expressing the SV40 Large T antigen. Have you made primers that work for QPCR? I tried 3 primer sets from the literature that did not...

25 February 2019 1,638 2 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

What analysis to choose with a large neste dataset and clearly skewed or kurtotic distributions?

Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...

02 March 2021 6,865 1 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

How to plot the probability paper of Gumbel distribution using R programing?

In R-studio, there are many commands of Gumbel package. Arguments are also different. I`m asking about the alpha parameter of the Copula which must be greater than 1. If this is the one used to...

25 February 2021 5,229 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View