Metaproteomic database for paper sequencing results?

More Miguel Angel Fernández-Martínez's questions See All

Tissue homogenization in mouse with FastPrep-24™ 5G - is the D matrix system useful?

I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...

31 July 2024 9,186 0 View

Can I use DNA a ladder for RNA samples in agarose electrophoresis? Or It is preferable to use cDNA amplified from RT-qPCR for the same samples?

I performed a nucleic acid extraction using 2 protocols for RNA extraction (Dengue Virus) and one parameter I need to evaluate is the integrity of my RNA samples. In this case, I can't use a RNA...

09 July 2024 4,857 2 View

Would the concentration of CFSE+ particles in EV samples be a more accurate way of measuring EV concentration?

Today, the most commonly used techniques for assessing the concentration of EV samples are NTA and the determination of total protein concentration. However, the concentration obtained by NTA...

16 May 2024 6,910 1 View

Agricuture, Innovation and Sustainability, Hot to encourage adoption and difussion in Spain ?

Which is the actual Status of Precission farming and regenerative farming in Castilla y Leon, Spain, which are the drivers that encourage adoption, and the potentil incentives, may catbon...

12 April 2024 7,901 0 View

How to determine variance of measurements between tests for each sample individually?

Hi, I am hoping to get some help on what type of statistical test to run to validate my data. I have run 2 ELISAs with the same samples for each test. I did perform a Mann-Whitney U-test to...

25 March 2024 7,474 4 View

?que imagen tiene el tulipán de un árbol de filogenético?

necesito una imagen de un filograma o árbol filogenético de un tulipán

23 February 2024 7,673 0 View

?imagen de un árbol filogenético de la flor la risa canina?

Necesito una imagen de un árbol filogenético de la flor de la rosa canina

22 February 2024 3,477 0 View

Can intracellular proteins fused to GFP be detected by flow cytometry?

If cells were transfected with a plasmid containing a gene of interesed (the resulting protein is intracellular) fused to GFP, would the GFP signal be detected by flow cytometry? Or can we only...

19 January 2024 888 1 View

What is the gold standard ratio for phenotype:healthy control for EHR phenotyping?

Hello! I am working on a proposal for creating an electronic health record phenotyping classification algorithm (mental health focus). I am having a hard time finding solid guidance re:cohort...

17 January 2024 8,856 2 View

Does Mannose 4B-CL Agarose From AG Scientific work?

I plan to purify a bacterial lectin (RSL) by affinity chromatography using mannose-agarose resin. I have found some mannose-agarose from AG Scientific (cat. number M-2797-10ML) that is almost 10...

08 January 2024 5,480 0 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

I'm optimizing a tetra-ARMS PCR protocol with amplicon sizes: 165bp, 123bp and 93bp. Why, in the gel, only 165bp is visible while I'm expecting all 3?

It's an end-point PCR protocol. I'm using 1.5% agarose gel with SyBR Safe dye and TBE as a running buffer, visualization on BioRad XR+ system. I was primarily thinking of primer efficiency,...

01 August 2024 4,673 4 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View

How to retain amplicon yield after Ampure bead cleanup, following a 2-stage PCR protocol?

Hello all, I have been trying to follow a 2-stage PCR protocol used to amplify barcodes of a large yeast library, as per Nyugen et al. (2022) -...

30 July 2024 841 2 View

Are the apoptotic cells is positive for γH2AX ?

It has been documented that apoptotic cells themselves can induce phosphorylation of serine 139 on H2AX (γH2AX) due to DNA fragmentation during apoptosis (doi: 10.1074/jbc.275.13.9390). As γH2AX...

28 July 2024 7,983 2 View

Can anyone help me explain my problem?

Hi, Now I am doing a single-base editing by using HiFi Cas 9, guideRNA, single stranded DNA, HDR Enhancer V2 and my tranfection mehtod is lipofection (RNAiMax). Unfortunately, after gene editing,...

26 July 2024 2,136 0 View

Hassan Nima

hope this link is useful

https://www.sciencedirect.com/science/article/.../S016816561731497...

regards

Miguel Angel Fernández-Martínez

Thanks for your response, Hassan.

However, I am not able to access the link.

Would you mind to send it again? Maybe something is lost after the copy/paste...

this link is useful

https://www.ncbi.nlm.nih.gov/pubmed/28663049

best regards

Khalid Al-Salhie

Interesting