Special issue on PLS-SEM in Mathematics. Are you interested?

21 February 2021 5,601 3 View

In opensees is the ReinforcingSteel material sensitive to units of measure?

20 January 2021 7,043 2 View

Could you help with a doubt regarding UDF velocity inlet profile with multiphase mixture (Ansys Fluent)?

We are two undergraduate students seeking for help!!! We are trying to simulate a laminar flux through a 3D pipe (25 cm long, 2 cm of diameter) consisting of a multiphase flux (air and water). We...

23 November 2020 3,918 3 View

How to generate pulsed laser from CW laser?

I was wondering if it could be possible to modulate a CW laser to pico/nano seconds pulsed laser somehow. I need to generate that condition to pump DFB polymeric lasers. Probably using...

16 November 2020 7,601 3 View

Which dose do you expose RAG1 deficient mice for lethal-irradiation?

Hello, I'd like to apologize for my English in advance, I'm still trying to polish it. We are performing several bone marrow transplantations (BMT) or fetal liver transplantations (FTL) to...

05 November 2020 4,239 1 View

How can I obtain the cohesive energy density and solubility parameter for a crystal using the Materials Studio software?

Hello, I am currently analyzing properties of some minerals such as Bi2O3 by MD using Materials Studio, I need to obtain the cohesive energy density (CED) to obtain the solubility parameter,...

02 November 2020 4,356 1 View

Grounded theory or Interpretive phenomenological analysis? Which is best for dissertation studying perinatal mental health?

I am curious what the differences are between using grounded theory or interpretive phenomenological analysis (IPA) for my dissertation. I am seeking to both complete my dissertation for...

29 October 2020 3,066 4 View

How to calculate grain size if FWHM of Lorentzian contribution is nearly zero and lower that FWHM of instrument?

I want to calculate grain size and microstrains. I fitted my peaks with a Voigt function but the FWHM of the Loretzian contribution resulted to be nearly zero, lower than the FWHM obtained from...

28 October 2020 2,578 1 View

How to validate CNVs (deletions and duplications) in human samples by qPCR?

Hello, I am interested in the validation of CNVs (deletions and duplications) in human samples by qPCR. We are trying to validate the primers for the experiments, but the primers are not working...

21 October 2020 5,896 2 View

How to convert saturated fatty acids into unsaturated fatty acids?

Suppose you have a mixture mostly comprised of saturated fatty acids of carbon chain length C10-C20. Is there a process to convert them to unsaturated fatty acids? The opposite process is well...

11 October 2020 2,955 4 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View

What might be the reason for obtaining two bands in plant genomic DNA extraction?

I got a thin band and a thick band i guess it would be the genomic DNA and the 260/280 ratio is 3.

21 February 2021 6,523 6 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View

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17 February 2021 4,217 3 View

Alternatives to traditional cloning of insert into backbone (dsDNA)?

Hi, I am stuck with a cloning experiment: The insert & backbone(bb) share one compatible end at 3' end(NotI) and one incompatible end at 5' end (XbaI for insert; HindIII for backbone). So i...

16 February 2021 1,664 3 View