Recombination events in confetti mice?

Dear all, I have been reading about recombination in confetti mouse strain (Brainbow 2.1), but I am not familiar with them, and quite confused how these events happen precisely. Most of the...

02 March 2019 8,123 14 View

H&E and Acian blue - nulcear fast red protocols for frozen section?

Dear all, I am planning to do histology on frozen sections of hard tissues (mouse bones). I treated the samples as following: 4% PFA for 4h, then sucrose gradients (10%, 20%, 30% with 24h each),...

10 November 2018 2,403 2 View

Isotype controls for IHC?

If I stain for the same type of tissue, but it is taken from animals at different ages, is it necessary to have age-matched isotype controls for each stained section? I wonder if aged-matched...

09 October 2018 340 5 View

Crystal violet staining for cartilage?

I would like to obtain mitotic figures of chondrocytes, so I am staining cartilage tissue embedded in paraffin with crystal violet. I would be very thankful if anyone kindly shares his protocol or...

04 May 2018 6,551 4 View

Can you share your experience in osteogenic differentiation of MSCs?

06 July 2016 8,484 8 View

Primers for Collagen type I alpha 2?

05 June 2016 1,942 3 View

How does siRNA function in response to viral infection?

10 November 2014 9,270 6 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

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02 March 2021 3,060 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

What analysis to choose with a large neste dataset and clearly skewed or kurtotic distributions?

Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...

02 March 2021 6,865 1 View

What is the characteristic length to determine the Reynolds number for the scattered turbulent fluid flow?

Dear all, I just saw this example in FB on the difference between laminar flow and turbulent flow. The flow from a pipe hits the sink and scattered. The flow from the pipe is characterized as...

02 March 2021 1,999 5 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View