8 Questions 23 Answers 0 Followers
Questions related from Mai Dan Nguyen
Dear all, I have been reading about recombination in confetti mouse strain (Brainbow 2.1), but I am not familiar with them, and quite confused how these events happen precisely. Most of the...
03 March 2019 8,291 14 View
Dear all, I am planning to do histology on frozen sections of hard tissues (mouse bones). I treated the samples as following: 4% PFA for 4h, then sucrose gradients (10%, 20%, 30% with 24h each),...
11 November 2018 2,474 2 View
If I stain for the same type of tissue, but it is taken from animals at different ages, is it necessary to have age-matched isotype controls for each stained section? I wonder if aged-matched...
10 October 2018 410 5 View
I would like to obtain mitotic figures of chondrocytes, so I am staining cartilage tissue embedded in paraffin with crystal violet. I would be very thankful if anyone kindly shares his protocol or...
05 May 2018 6,627 4 View
Hi colleagues, I'm studying the osteogenesis of MSCs. I used some cell lines before, so I don't have much experience with primary culture of MSCs. Would you mind suggesting some protocols for...
07 July 2016 8,562 8 View
I'm now trying to induce osteogenesis of C3H10T1/2 cells, and I'm going to analyze gene expression of the gene Collagen type 1 alpha 2 using qPCR (only SYBR is available in our lab). However, it...
06 June 2016 2,011 3 View
siRNA could lead to the degradation of a target mRNA, but I could only understand in the case that this target mRNA synthesized by the host cell itself; thus, how can siRNA act as a natural...
11 November 2014 9,397 6 View
In flow cytometry, what kind of marker is popularly and effectively used to identify neural stem cells isolated from embryonic rat brains? And if I used a neurosphere assay, including the...
12 December 2012 4,071 0 View
