How do I get protocol to isolate Dithymoquinone from Nigella saliva (black seeds)?

Good day scholars. Please I need detail protocol on how to isolate dithymoquinone from black seeds. I intended to buy dithymoquinone directly but what I kept getting is thymoquinone. Hence,...

02 December 2020 4,062 1 View

Why is Thymoquinone preferred over Dithymoquinone?

Thymoquinone and Dithymoquinone are found in black seeds (Nigella sativa). From their structures, dithymoquinone has 2 rings while thymoquinone has 1 ring. One would expect that dithymoquinone...

02 December 2020 303 2 View

Can I plot Bar Chart with significance levels represented by alphabets in R?

Good day house. Please can I plot Bar Chart with significance levels represented by alphabets in R? A file is attached to show the graphical representation of my question. I came across this bar...

15 October 2020 7,282 4 View

How do I determine Gestational Day 0 in Rats and Mice to start treatment?

Good day house. Please I intend to start exposure and treatment in Rats and Mice on the first day of pregnancy. So how do I determine the first day of pregnancy which is always designated as...

15 October 2020 7,402 4 View

'vina: command not found' error in virtual screening using autodock vina?

I am trying to carry out virtual screening using autodock vina. I have all the files necessary in my directory as suggested by literatures and tutorials. But when I run the command ./vina_vs.sh,...

22 September 2020 3,859 3 View

Is there R package and code to calculate MEAN +/- SEM?

Good day house. Please is there R package and code to calculate MEAN +/- SEM? I know of MEAN +/- SD, but I have not come across MEAN +/- SEM. Besides, is it possible to generate data set given...

05 April 2020 3,842 3 View

Can glass ionomer cement be used for cementing ceramic veneers as an alternative to resin cement?

I read an article which stated that resin modified glass ionomer cement (RMGIC) cannot be used for cementation of ceramic veneers due to development of hygroscopic expansion leading to fracture...

11 March 2020 3,684 4 View

What can made DNA/PCR product disappear after Cleaning?

I extracted gDNA from blood samples preserved on FTA paper using QIAamp DNA mini Kit and then ran PCR. The Agarose Gel Electrophoresis reveal expected band. To clean my DNA before Sequencing, I...

11 April 2019 5,534 3 View

Which DNA Sequence Statistics should I do?

Good day house. I am working on Molecular Evolution and Phylogenetics of some organisms. Before conducting my Synonymous to Non Synonymous substitutions after Sanger Sequencing, I would need to...

08 April 2019 5,039 3 View

What is the function of the Universal primer, Inosine, in an oligonucleotide primer?

Good day house. Please what is the function of the universal primer, Inosine, in a primer? I need to use the primer for PCR. If I don't include the Inosine in the primer as published what is the...

12 March 2019 6,672 4 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View