How do I get protocol to isolate Dithymoquinone from Nigella saliva (black seeds)?

Good day scholars. Please I need detail protocol on how to isolate dithymoquinone from black seeds. I intended to buy dithymoquinone directly but what I kept getting is thymoquinone. Hence,...

02 December 2020 4,062 1 View

Why is Thymoquinone preferred over Dithymoquinone?

Thymoquinone and Dithymoquinone are found in black seeds (Nigella sativa). From their structures, dithymoquinone has 2 rings while thymoquinone has 1 ring. One would expect that dithymoquinone...

02 December 2020 303 2 View

Can I plot Bar Chart with significance levels represented by alphabets in R?

Good day house. Please can I plot Bar Chart with significance levels represented by alphabets in R? A file is attached to show the graphical representation of my question. I came across this bar...

15 October 2020 7,282 4 View

How do I determine Gestational Day 0 in Rats and Mice to start treatment?

Good day house. Please I intend to start exposure and treatment in Rats and Mice on the first day of pregnancy. So how do I determine the first day of pregnancy which is always designated as...

15 October 2020 7,402 4 View

'vina: command not found' error in virtual screening using autodock vina?

I am trying to carry out virtual screening using autodock vina. I have all the files necessary in my directory as suggested by literatures and tutorials. But when I run the command ./vina_vs.sh,...

22 September 2020 3,859 3 View

Is there R package and code to calculate MEAN +/- SEM?

Good day house. Please is there R package and code to calculate MEAN +/- SEM? I know of MEAN +/- SD, but I have not come across MEAN +/- SEM. Besides, is it possible to generate data set given...

05 April 2020 3,842 3 View

Can glass ionomer cement be used for cementing ceramic veneers as an alternative to resin cement?

I read an article which stated that resin modified glass ionomer cement (RMGIC) cannot be used for cementation of ceramic veneers due to development of hygroscopic expansion leading to fracture...

11 March 2020 3,684 4 View

What can made DNA/PCR product disappear after Cleaning?

I extracted gDNA from blood samples preserved on FTA paper using QIAamp DNA mini Kit and then ran PCR. The Agarose Gel Electrophoresis reveal expected band. To clean my DNA before Sequencing, I...

11 April 2019 5,534 3 View

Which DNA Sequence Statistics should I do?

Good day house. I am working on Molecular Evolution and Phylogenetics of some organisms. Before conducting my Synonymous to Non Synonymous substitutions after Sanger Sequencing, I would need to...

08 April 2019 5,039 3 View

What is the function of the Universal primer, Inosine, in an oligonucleotide primer?

Good day house. Please what is the function of the universal primer, Inosine, in a primer? I need to use the primer for PCR. If I don't include the Inosine in the primer as published what is the...

12 March 2019 6,672 4 View

How to analyze a particular region of my simulation box in GROMACS?

Hi, I am using GROMACS 5.1.4 to simulate water in contact with a surface. I am only interested to calculate some properties of water near the surface. Let's say at a distance of x nm from the...

03 March 2021 9,989 2 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

How to get onecolumn document from standard IEEE ACCESS LateX template?

I have prepared a manuscript in IEEE Latex template, but for some purpose, I need to prepare a word file as well (detailed format is not required). Compared to two column .tex file, one column...

02 March 2021 1,830 3 View

How can the modification of garden soil cause global warming?

Hello, I am working on a soil project and I found a primary research article that tests how the modification of agricultural soil can cause global warming, using a thermal collector, however I...

02 March 2021 7,161 3 View

Does anyone know how to plot my station to a section plot on Ocean Data View (ODV)?

I made a vertical section plot with WOA .nc file on ODV, and now I want to plot my stations (csv. file) in it. Does anyone know how to import my points to the section? They are two different...

01 March 2021 3,610 1 View

Does anyone know to clarify these changes?

01 March 2021 1,290 2 View

How can I convert .vesta file to a .cif file?

I've used the "export data" option in Vesta software to convert my unit cell into a cif file however when I use that cif in atomsk it shows error such as "can't read the position of atom 0". If I...

28 February 2021 4,966 3 View

How can we use a hybrid element in Abaqus Dynamic/Explicit for impressible materials?

Hi, all! How can we use a hybrid element in Abaqus Dynamic/Explicit for impressible materials? I have not find the choice for explicit element in Abaqus CAE. I am wondering whether it is possible...

25 February 2021 9,936 2 View

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25 February 2021 5,713 1 View

Anyone that can help with Maxquant TMT16plex which is missing?

Hi I am very new to proteomics analysis. I have got raw files from core facility running thermofisher TMT 16plex 4 hours. I cannot find on maxquant the option TMT16Plex there is only up to 11...

25 February 2021 1,675 3 View