How do open a vcf file containing some sequences?

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Could you please help me identify what might be wrong with my plaque assay?

I used Vero E6 cells and Mayaro virus. The virus was diluted in DMEM without FBS. The plaque media was 2X MEM, prepared with sodium bicarbonate and L-glutamine, adjusted to a pH of 4 with sodium...

03 June 2024 519 0 View

What is Open education resources (OER)?

As a modern and digital teacher, how can you identify and evaluate them for usage in teaching and learning process

19 April 2024 1,218 1 View

How can I turn the map I created (the .shp file) into an interactive map?

After collecting coordinates and mapping the road network, how do I then turn my created map into an interactive map

10 April 2024 3,659 1 View

What is the best agro waste used for mitigating corrosion? WHY?

Various agricultural residues have been employed for corrosion testing. Which specific agricultural residues are optimal for testing under a particular analysis?

28 December 2023 6,550 1 View

Is it possible to get a postdoctoral study after 5 years of obtaining a doctoral degree?

Most postdoctoral openings usually state that applicant's doctoral degree should not have exceeded 5 years before application. I need a possible list of University/Establishment that could admit...

03 July 2023 1,729 2 View

What can I use as a positive control for endothelial cell proliferation (MTT assay)?

Planning to run my assay this week but couldn't figure out positive control for MTT assay. I want to use cells without treatment as negative control while needing an agent or growth factor that...

12 March 2023 1,313 14 View

Is it possible for urea conc in a batch study to increase with time. The case that the urease enzyme should be hydrolyzing the urea?

I recently did a batch study with S.pasteurii and yeast extract plus some urea solution containing 20g/L urea. At 0hr sampling the urea conc was 13g/L, at 4 hrs sampling was 17g/L then it was...

18 July 2022 4,183 2 View

Can anyone help with a spectrophotometry method/protocol for determining dopamine level in rats' 🧠 brain?

Good day scholars. Please I need to determine the dopamine level in the brains 🧠 of experimental rats 🐀. I don't want to use ELISA; the alternative method I got requires spectroflurimeter which I...

10 June 2022 6,758 0 View

How do I determine B-Amyloid and NO in brain homogenate?

Good day scholars. Please can anyone help with detailed protocol(s) for the determination of Beta-Amyloid, dopamine, Nitric oxide (NO), Monoamine oxygenase A (MAO-A), and Acetylcholine esterase...

08 August 2021 2,357 1 View

Can anyone help with Tail cuff to meausre blood pressure on rats?

Good day scholars. Please I need to measure the blood pressure of rats at interval. I will appreciate if anyone can help with link to get this tail cuff/monitor?📱

25 July 2021 8,436 2 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

How do I replace a file with a more recent version of a paper that was uploaded to ResearchGate?

The paper in question is "Interpolation of Nitrogen Fertilizer Use in Canada from Fertilizer Use Surveys". This paper was very recently published by Agronomy (MDPI). Agronomy has, in the last day...

07 August 2024 9,934 3 View

How to solve g_mmpbsa error?

Program: g_mmpbsa, version 2024.1 Source file: extrn_apbs.cxx (line 152) Fatal error: Failed to execute command: $APBS pybYcUWA.in --output-file=pybYcUW.out

07 August 2024 6,066 0 View

How do you delete a duplicate pdf for the same paper on ResearchGate?

The first pdf file I uploaded had an error. So I uploaded an updated, corrected pdf of that paper with a different pdf name. I dpon't want the old copy to be download or read.

07 August 2024 9,508 1 View

AUX gas reading problem on QE with full MS and PRM method in one run?

Dear QE-users, In the method where full MS positive mode and PRM mode are used, we always get an incorrect auxiliary gas reading (41 instead of 25). This only happens in this method; other...

06 August 2024 4,953 0 View

Why did the authors extrapolate a phenotype that they experimentally proved in one bacterial strain across the whole genus of the organism?

I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...

05 August 2024 6,787 4 View

JCPDS 65-7246 file please?

Dear Researchers Kindly share JCPDS 65-7246 file Thanks in advance

04 August 2024 5,613 1 View

Dirty and clean?

Hi everyone I need a file with a dirty and clean potato image

04 August 2024 7,199 4 View

How to change the version of the article full-text pdf file?

How to change the displayed full article text to its corrected version? In the file on the page of the journal where I published the article, there was an error in the text, the table is...

30 July 2024 3,229 2 View

Difficulty with permittivitt and Magnetic Permeability Calculations?

Difficulty with permittivitt and Magnetic Permeability Calculations Hello everyone, I have all the parameters related to the calculations of the permittivitty and magnetic permeability...

30 July 2024 5,206 1 View