Does this your research project to synthesis a lyse solution for the granulocytes?

Do you want to lyse the erythrocytes from whole blood in order to purify the granulocytes/neutrophils, or lyse the granulocytes?

One common approach involves using a hypotonic solution combined with detergents to disrupt cell membranes rapidly. Here's a simple recipe for a lysis solution:

Ingredients:

1. Distilled water (hypotonic)

2. Detergent (such as Triton X-100 or NP-40)

3. Salt (optional, for maintaining osmolarity)

4. Buffering agent (optional, for pH stability)