I want to measure protein content in my plants. So, ı will use Lowry method. How can ı prepare extraction buffer for plant materials?
Do you have a tried protocol for GSH measurement? I will use it for wheat.
17 September 2023 2,984 0 View
I use the acetone method for chlorophyll analysis. Should I do homogenization after making an addition with acetone?
09 September 2023 6,533 2 View
Let (X, tau, I) be an ideal topology I ask: wThe product of this topology itself is ideal topology or not?
21 August 2023 6,122 4 View
I'm going to measure chlorophyll spectrophotometrically. Does anyone have a tried-and-tested, detailed protocol?
14 July 2023 4,910 4 View
Can I write codes for ridge quantile regression in R package program? Are there any resources for this? What are your recommendations? Thank you for your answers in advance.
29 May 2023 7,046 3 View
I use the Ellmann method for the determination of glutathione. There are so many options as a buffer. I prepared a 0.1 molar 8.4 pH Tris buffer for the reaction mixture. I have a trismza base...
23 May 2023 2,501 2 View
I am trying to simulate an hypervelocity impact analysis between spherical projectile and composite fabric including fiber and matrix. (All of them are SPH) Elastic plastic hydro material and...
12 May 2023 3,971 1 View
I am trying to simulate an hypervelocity impact between a SPH sphere and FEM composite material which is a combination of fiber and matrix. After the impact instead of damage or erosion i obtain...
12 May 2023 8,020 0 View
I did a GSH measurement on the samples today in -80. I used the Ellman method. Reaction comparison: 0.1 ml sample, 2 ml Tris 8.4, 0.1 ml Ellmann reaction. i measured it at 412 nanometers, but my...
13 April 2023 7,633 2 View
I'm going to measure the GST enzyme activity spectrophotometrically. Does anyone know the procedure? The absorbances I measured turn out to be at a negative value. I used the Habig method.
23 January 2023 5,204 3 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,081 3 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,881 2 View
Does anyone tried to do nucleofection with AMAXA by Lonza with lower than recommended amount of buffer in the cuvettes (100 ul)?
07 August 2024 4,614 0 View
Currently, when I run SDS-PAGE, I don't see any bands at all, even though I used the same material just a day ago and it worked fine.... In our lab, we dilute the 10X running buffer to 1X and...
06 August 2024 5,372 2 View
Hello everyone, I'm encountering an issue with my electrochemical impedance spectroscopy (EIS) measurements and would appreciate some insights. Experimental Setup: Electrodes: Gold interdigitated...
05 August 2024 3,781 2 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 500 2 View
HOW CAN I WRITE A CODE TO USE THE WAVENET TRANSFORM AS A FEATURE EXTRACTION METHOD INSTEAD OF DWT IN MATLAB?
03 August 2024 7,827 0 View
Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC
01 August 2024 966 3 View
I am currently working on a project that involves extracting cell membranes, for which we disrupt the cells using sonication. During the initial extraction process, we add protease inhibitors to...
30 July 2024 7,077 1 View