Hi, we are currently doing a CRISPRi screen in THP-1 cells. Our first transduction is with TA3 lentiviruses. These carry Blasticidin resistance. The only way to collect these cells, and to distinguish live from dead cells, are to flow sort them as they are non adherent. We are looking for a viability reagent that is not cytotoxic, will not damage DNA of the cells, and the dye won't stay with the cells, as our next phase of transduction and sorting will be with mCherry.
Hi Abigail Courtright
In your case I think the most appropriate method is to detect then FITC annexin kit. This kit can distinguish live, apoptotic and dead cells by different colors. Its protocol is pretty simple.
For more information you can refer to the following link:
https://www.thermofisher.com/order/catalog/product/V13242
Best....
I think what you are looking for is calcein AM. You can read further here https://en.wikipedia.org/wiki/Calcein
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