Abigail Courtright

3 Questions 4 Answers 0 Followers

Questions related from Abigail Courtright

Looking for cell viability stains for flow sorting, any ideas?

Hi, we are currently doing a CRISPRi screen in THP-1 cells. Our first transduction is with TA3 lentiviruses. These carry Blasticidin resistance. The only way to collect these cells, and to...

01 June 2022 5,326 2 View

How do you ensure single integration of sgRNAs when doing a CRISPR screen?

I know I am looking for an MOI of 0.3, but I am trying to figure out how one can ensure this.

06 April 2022 1,467 2 View

What cell lines can I use?

I am looking for a macrophage human cell line. Preferably one that is already adherent vs a cell line that requires differentiation.

31 March 2022 758 5 View