How do you ensure single integration of sgRNAs when doing a CRISPR screen?

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COW Algorithm R Package for GC-FID Pre-processing?

Hi! Are there any R packages or Python modules that are able to implement the COW algorithm in chromatogram alignment? Thanks!

13 July 2023 1,926 0 View

How do you deal with negative values in predictor and independent variable in regression?

I am trying to run an OLS regression, with two continuous variables, both with negative and positive values. How should one deal with this? How do you plot a regression line in this case? Is it...

29 December 2022 5,945 4 View

I am running RegCM4.7.1 with ERA5, Terrain and SST was successfully but when I run ICBC, it returns:No timesteps for SST data in SST file. Why?

This is icbc part of the RegCM version 4 SVN Revision: 4.7.1-1027-g0c9c8 compiled at: data : Oct 8 2021 time: 04:29:07 : this run start at : 2022-12-30 04:39:43+0000...

29 December 2022 930 18 View

Recent issues with Sylgard 184 for microfluidics?

Hi, Has anyone doing microfluidics work for cell culture started having problems recently with getting their Sylgard 184 PDMS to reliably and permanently bond to the glass substrate? We have tried...

10 July 2022 7,822 1 View

Looking for cell viability stains for flow sorting, any ideas?

Hi, we are currently doing a CRISPRi screen in THP-1 cells. Our first transduction is with TA3 lentiviruses. These carry Blasticidin resistance. The only way to collect these cells, and to...

31 May 2022 5,325 2 View

What cell lines can I use?

I am looking for a macrophage human cell line. Preferably one that is already adherent vs a cell line that requires differentiation.

30 March 2022 757 5 View

Coding an Emotional Stop Signal Task in E-Prime 3.0 with variable stop signal delay?

Hello All! I am working on implementing an Emotional Stop Signal Task in E-Prime 3.0. Validation of an emotional stop-signal task to probe individ... Originally my plan was to modify the...

20 March 2022 1,658 1 View

Has anyone found the use of COVID-19 disposable masks increased microfiber contamination in microplastic lab samples?

I am an undergraduate student learning the ropes of microplastic sample processing and have found that my blanks continue to have above average counts of fiber contamination. We have found that...

18 October 2021 2,714 2 View

Know any gas chromatogram alignment software?

Hello! I have a lot of chromatograms with hundreds of peaks that need to be aligned. Can you recommend software (hopefully, opensource) that I can use to align peaks? Thanks!

10 September 2021 2,240 5 View

Does anyone teach psychobiography? In that case, any recommendations?

I am preparing a 5-7 hour-course about psychobiography and I would like to know how others train students in this field. Here in Spain it is unknown so I will be the one introducing the method. My...

18 June 2021 4,033 4 View

Can you connect an HPLC to a Mass Spec only at a certain time point?

Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...

11 August 2024 8,141 3 View

GC-MS retention index prediticon?

Hello experts, Does anyone know any free software about retention index prediction ?

08 August 2024 7,403 2 View

How to calculate CCS for Sodiated adduct ions and Multiply Charged Ions?

I'm currently working on calculating the collision cross section (CCS) for various ions, and I'm facing challenges when dealing with sodiated and multiply charged ions. Most of the resources I’ve...

08 August 2024 8,329 0 View

Where to find a gene list for CRISPRa/i library screening of regulatory factors that affect pathogenic Th17 differentiation in PBMC?

I want to perform a CRISPRa/i library screening for candidate regulatory factors that affect pathogenic Th17 differentiation in PBMC. But I am wondering how to define the size of gene library, so...

31 July 2024 2,150 0 View

Hello dear researchers. I would like to know what is a normalized mass in an EDX analysis?

EDX analisys

31 July 2024 5,947 2 View

Gas chromatography RT detection?

I am working on algal extract to which gas chromatography (Not GC-MS) spectrum I want to discover. My question is can we identify specific compounds using retention time if I compared the RT with...

29 July 2024 8,034 4 View

MAGeCK Plotting Error: Logarithmic Axis Must Have Positive Limits?

I have been running a MAGeCK test command on the terminal for a CRISPR screen to rank sgRNAs and genes based on the read count tables. However, I get only the plot of the top-ranked genes...

28 July 2024 9,811 1 View

Screening of EPS producer LAB?

if you have MRS broth already prepared, what is the manner you will follow for preparing hyper-sweet medium for screening of EPS producer LAB? what the concentration of saccharose or glucose you...

26 July 2024 6,606 0 View

Why knockouts for same genes from two different gRNA are showing different result?

Why knockouts for same genes from two different gRNA are showing different result? I followed CRISPR-CAS9 gene editing technology.

25 July 2024 8,313 1 View

Could international experts in media and journalism, AI, communications and international communication share this conference link?

"Integration of Mass Media and Communication into the System of International Relations: Place, Role, Actuality and Prospects: Modern Media Issues 2024" Website: https://immcir.alnoor.edu.iq/

23 July 2024 3,139 6 View