Can someone please explain the credibility behind Travelodge's survey: "The Secret to a Good Night's Slumber is to Sleep in a Blue Bedroom" ?

Currently, I'm investigating the effect bedroom color has on one's quality of sleep. Many (non scholarly) works I've come across have mentioned / cited this survey, conducted by Travelodge in...

21 September 2019 4,088 2 View

How do you get SPSS to provide confidence intervals for the standard error of estimate?

I would like to compare a test device against a criterion device. Regression analysis of z-scores in SPSS provides Pearson's correlation values (plus CI's) and a standard error of estimate, but it...

10 December 2018 7,994 1 View

I have over 66 published papers, not the single paper with 2 students? How could you miss them all?

I am looking for some recent papers on the Cordillera with a focus on paleomagnetic data.

11 March 2018 8,858 4 View

Which multiple comparison option is best for determining if combo therapy is better than either mono therapy?

In my experiment, I am attempting to determine if the combinatorial therapy (composed of therapy A and therapy B) is better than either alone. My experimental groups are as follows: negative...

17 January 2018 2,502 13 View

Not column based DNA extraction kit for stool/soil samples?

24 May 2017 6,814 3 View

Looking for a lab with an IMR-MS (Airsense) facility?

We want to measure breath isoprene on a small (20 ml) volume of collected breath but would like to collaborate/contract with a lab that has the analytical facility/expertise rather than buying a...

26 September 2016 3,078 1 View

Favorite program/app to organize digital images into a searchable/filterable database??

08 June 2016 3,850 2 View

Is there a human CTLA-4 Ab clone that is not reactive or functional?

We are isolating CTLA-4+ cells using Miltenyi immunomagnetic beads and are finding that the cells are massively dying on the column. We don't see the other subsets isolating so believe it to be...

06 February 2014 1,838 1 View

How to identify kinase (or phosphatase) that phosphorylates/dephosphorylates my protein?

I have a protein with a phosphorylation site on the extracellular surface of the membrane, and I would like to figure out which kinase is phosphorylating it, and if possible which phosphatase...

03 November 2013 6,631 3 View

Can Lipid A be observed on Western Blot or SDS-Page? Would it attach a monoclonal antibody or adhere to a dye?

Qualitative analysis of LPS

19 September 2013 7,090 3 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Research on Supply Chain Management in Indian context?

Hi, I am planning to apply for the PhD degree in the Supply Chain Mgt. with specific area of "Cold Storage warehouses" during Pandemics and wars. Where lock downs and shut downs are frequent....

02 March 2021 285 2 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

Expression cloning by using cDNA,do I need to modify the cDNA first?

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...

28 February 2021 5,440 3 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View