Kait Stone

6 Questions 4 Answers 0 Followers

Questions related from Kait Stone

Looking for a PCR to detect the pESI plasmid in Salmonella Infantis?

Salmonella surveillance

12 January 2018 2,963 3 View

Not column based DNA extraction kit for stool/soil samples?

I'm looking for a DNA extraction kit that is beed based. So, far I found only one from Omega. Do I have any other options? Please advice.

25 May 2017 6,897 3 View

Can Lipid A be observed on Western Blot or SDS-Page? Would it attach a monoclonal antibody or adhere to a dye?

Qualitative analysis of LPS

20 September 2013 7,163 3 View

Does anybody know the effect of aluminium hydroxide (which is bound to the antigen) on protein extraction and/or protein separation on PAGE?

There should be lots of proteins in the vaccine (in oil adjuvent) as we can observe millions of cells with Gram Stain; however, only a few protein bands showing up on Western Blot. Could...

06 June 2013 250 0 View

Resolving proteins from a vaccine?

Does anybody know a good way to see proteins in a vaccine? I see only a couple of bands running protein gels from a vaccine, while I see lots of proteins on simply bacterial cells. What effect do...

21 May 2013 1,721 1 View

How to revive hybridomas?

I'd like to receive some input from people who worked with hybridomas. I need to resurrect them from being stored at -70C for about 5 years. I've never worked with hybridomas before and would...

17 January 2013 9,319 2 View