01 March 2021 3 6K Report

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli

However I don't know I should only inserting the Coding sequence ,or I can just insert whole cDNA into the plasmid and let the bacteria express.I am curious whether the 5' and 3' UTR will express or not,and if they express ,will they change my interested protein structure? Thank you so much

More Joe Li's questions See All
Similar questions and discussions