How can I simulate noise of CMOS Image Sensor by sentaurus TCAD?

Can anyone tell me how to simulate noise of CMOS image sensors, such as thermal noise and flicker noise with sentaurus TCAD?

09 November 2020 9,616 4 View

How to stain iPSC derive motor neuron in 96-well plate without detachment?

Hi all, I am using 96-well plates to culture iPSC derived neurons, the problem is, when doing ICC, my neurons tend to detach during the washing procedure, till the final step nearly all neurons...

14 July 2020 3,828 7 View

Can I put one mouse in one cage?

I have three mice but I only need to scarify two in my experiment. Can I keep the leftover one mouse in the cage with itself? The other option is to put it in another cage with other mice. But...

12 July 2020 307 5 View

Why ammonium chloride an inhibitor for lysosome?

Ammonium chloride is a lysosome inhibitor and a weak base as described in a published paper, Ammonium chloride, an inhibitor of phagosome-lysosome fusion... . However NH4+ is a weak acid, I...

21 April 2020 8,419 3 View

How to design steel rack accorfing to ANSI MH 16.1: 2012?

Does some have got the commentary of the American norm ANSI MH 16.1: 2012? according to this norm the seismic design of the steel rack could be performed using R=4 as behavior factor, this value...

19 November 2019 6,598 2 View

Why I have very strong phospho protein signal in western blot?

I am working with phospho protein in western blot and have problems about the phospho protein signal. It is common that researchers can not detect phospho-protein signal in the membrane, as...

17 July 2019 6,027 3 View

How to decontaminate a 2D materials on TEM grid for atomic scale imaging?

I was trying to imaging the 2D materials with aberration corrected TEM, including MoS2, ReS2 and graphene. However, I found serious carbon contamination when zoom in at the region of interest....

30 May 2019 9,828 3 View

How to use software Geneious to find genomic features?

How to use software Geneious to find genomic features such as genome size, GC content, tRNA genes, CDS etc?

10 June 2017 1,062 1 View

How canI verify the DNA fragment without results after blast in NCBI?

15 January 2017 4,595 3 View

Does anyone have any reference about only using fluent and ansys workbench static structural to do a two way fsi analysis for a propeller?

Can anyone give me a hand to share some reference about this problem and I will be very thankful for that.

05 February 2016 6,931 3 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View

Can thermal cycler machines be problematic during PCR?

Hi, my question is about the heating of thermal cycler machines and I hope some of you had experienced a similar thing previously. There are two thermal cycler machines in the lab(BioRad) and for...

26 February 2021 4,777 4 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View