Long-amplicon real-time PCR?

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Giorgia Pertile

My opinion try to draw the primer to amplify a specific region of 1 kb. If you try to amplify this big product your efficiency decrease much.

Bingqian Qu

Thank you. Whether decreased efficiency is related to unavailability of DNA polymerase used in real-time PCR technique?

@bingqian Qu

The efficiency of the qPCR is connected at more factors.For example to the Tm of the primers, concentration of DNA, concentration of primers, number of cycles, inhibitors inside the DNA samples.