Please help me with this question?

In a heat exchanger, the cold stream is a corrosive material and it flows through the tube side. The shell side fluid is at a higher pressure than the tube side_ Recently one of the tubes in the...

01 December 2020 2,860 6 View

Help for data analysis?

I am working with raster data for ANN, i have converted rasters to numaric and then test train that numerical data and get very Good accuracy. now i need to convert my train data into raster using...

14 November 2020 9,902 2 View

Please share your ideas and research for future PhD candidates in Meteorology research direction?

Hello Respected Researchers, I'm Looking forward to the ressearch community to share ideas and research work for future PhD candidates in Meteorology Direction related to Hydro Meteorology, remote...

22 June 2020 3,475 16 View

Share your ideas for future PhD candidates in Environmenal Sciences research direction?

Hello Respected Researchers, I'm Looking forward to the ressearch community to share ideas and research work for future PhD candidates in Environmenal Research Direction related to remote sensing,...

21 June 2020 1,539 28 View

Why I am observing a voltage drop from 2.4 to O V while discharging Lithium Sulfur battery?

I made a Lithium sulfur cell, with Sulfur/Carbon cathode and Lithium metal anode using 100 uL of electrolyte (0.6 M LiNO3 and 0.4 M LiTFSI in DME:DOL). I am using a Biologic VSP 300 battery cycler...

27 May 2020 1,232 5 View

Can anyone please suggest me a free software for developing Forest Plots for meta-analysis?

Currently, I am using STATA 13 but the graphical output is not as good as presented in papers (meta-analysis) published in some reputable journals.

28 April 2020 7,979 5 View

Any useful softwares for applying neural networks in power systems?

Any useful software for applying neural networks in power systems? I know MATLAB has a NN toolbox but besides that, is there any other useful software available?

20 April 2020 9,663 16 View

Any suggestions on where to begin learning neural networks for beginners?

Any suggestions on where to begin learning neural networks for beginners? Some advice on useful resources , books, papers, etc will be appreciated. Thanks

18 April 2020 8,153 13 View

Any suitable index for describing power system frequency stability under a three phase fault?

Any suitable index for describing power system frequency stability under a three phase fault?

01 April 2020 9,204 12 View

What is DPL command to find maximum value in a graph drawn in Digsilent Power Factory?

what is DPL command to find maximum value in a graph drawn in Digsilent Power Factory?

28 March 2020 5,674 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View