Overview of Machine Learning. What are the opportunities it brings to developers? What threats does it pose to society?

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Does anyone know, can i do cryosectioning of whole aorta after oil red O staining and use the cryosection parts for further analysis?

I would like to quantify the lesions on aorta. I want to take image of atherosclerotic plaques formation in aorta by using O red Oil Solution. After the staining, what will be the storage...

16 February 2021 5,876 2 View

What is the best concentration of Pam3CSK4 to coat a dish to activate with THP-1?

Hi everyone I am trying to coat a glass-bottom dish with Pam3CSK4 and plate THP-1 cells to measure IL6 with ELISA from the supernatant. I wondered if anyone knows a good concentration and...

21 January 2021 2,593 1 View

Why I see Lamp-1 GFP in cytocol after transfection?

Hi every one I transfected Lamp-1 GFP plasmid to the HEK293 cell line. When I image the cells in TIRF, I see green fluorescence o the cytosol too. Does anyone have any idea that how it can...

21 December 2020 9,978 3 View

Why HEK293 transfected with LAMP-1GFP do not load dextran?

Hi every one I have made a cell line of HEK293 transfected LAPM-1GFP and after seeding the cells for 24h, I try to load dextran on the dish but after imaging, the cells with GFP+ have loaded less...

14 December 2020 1,801 2 View

What are the tools or software which can simulate climate change for a city and conclude future climate data?

I am working in research on climate adaptation so need to compare the performance of the urban fabric to the climate changes, which requires me to make a comparison and simulation of the...

03 December 2020 988 8 View

Please help me with this question?

In a heat exchanger, the cold stream is a corrosive material and it flows through the tube side. The shell side fluid is at a higher pressure than the tube side_ Recently one of the tubes in the...

01 December 2020 2,860 6 View

Correlation, Regression and significance?

Dears, I have different variables in multiple regression. Two variables are highly correlated. To solve the collinearity issue, I dropped one of them from the multiple regression model. The...

29 November 2020 2,695 4 View

Do anyone have any authentic material regarding Cultural Ethics and especially regarding Pakistani culture?

I need this because I have to represent it in a conference as a keynote speaker

17 November 2020 584 4 View

How can I synthesis heparin nano particle?

I want to synthesize iron nanoparticles with heparin, but I have trouble synthesizing First of all, do I have to use a freezer drying for drying of product and it is not impossible use from...

16 November 2020 9,455 2 View

Help for data analysis?

I am working with raster data for ANN, i have converted rasters to numaric and then test train that numerical data and get very Good accuracy. now i need to convert my train data into raster using...

14 November 2020 9,902 2 View

How to add missing citation?

Dear colleagues, I have found that many authors have a concern regarding their missing citation in RG because often citation in RG is lower than google scholars. I also missed some citation...

26 February 2021 928 6 View

What is your preference for journals for publishing in the field of Hyperspectral Imaging or Remote sensing?

I believe that we need to share our experience in publishing in the field of Hyperspectral Imaging or remote sensing? What is the best journal that you publish with? What is the worst experience...

23 February 2021 3,411 4 View

Can we take 96 well plate readings in three different plate readers ( different companies) for 12 hr,24hr and 48 hrs? With same 540nm wave length ?

My experiment which require 12hr,24hr and 48 hrs readings at 540 nm wavelength with referenc 560 nm wave length after mtt assay and dmso treatment . Can i take readings of 12 hr ,24 hr and 48 hrs...

09 January 2021 5,989 2 View

Are international conferences a dying species?

In our discipline (chemistry), annual meetings of the American Chemical Society (ACS) used to be mega-events with up to 20,000 participants. The current one will be virtual, and the organizers...

06 January 2021 1,882 5 View

Could you name a few country (developing) worst affected due to corruption?

The impact of corruption in education is unbearable, as witnessed out from the sufferings of society in all different aspects of life. Can you please provide a comparative impact on the percentile...

03 January 2021 2,725 8 View

Homeopathy is within the reach of most of the financial class?

Greetings. I personally have taken homeopathy medications and found it effective in curing. I do not know if it is effective in all kinds of diseases, but it is within the financial reach of all...

02 January 2021 9,904 5 View

Which is the best referencing tools ,website...?

We get difficult in referencing, and not getting any reliable site

22 December 2020 9,386 4 View

Experimental Data Set of Sluice Gates Under Free Flow Regime?

Could anybody share a dataset for the sluice gates operating under the free-flow regime? I need the upstream and downstream depths as well as the opening of the sluice gate at least. Any help...

17 December 2020 4,405 1 View

Is there any paper related to solar radiation prediction or photovoltaic power generation from IEEE geoscience and remote sensing society?

I have been looking for some paper related to solar radiation prediction or photovoltaic power generation from IEEE Geoscience and Remote Sensing Journals ( IEEE Transactions on Geoscience and...

08 December 2020 1,117 3 View

Is it acceptable to publish data that were digitized from a previously published plot/figure (referencing the original figure of course)?

There are many ways to extract original source data from figures and plots in publications. If one were to do that and reanalyze the data in the same or a different way, is that acceptable in a...

28 October 2020 3,822 2 View