What is the minimum input concentration & read depth & coverage required for single cell DNA methylome study either by WGBS or RRBS?

11 January 2016 9,324 1 View

Is anyone familiar with the green disposal of both organic and inorganic wastes in bulk, of a Pharmaceutical Industry?

08 April 2015 1,556 3 View

How do we quantitate RNA from a single cell?

For RNA obtained from a single cell not only the low concentration demands a highly sensitive instrument, but also the volume of total RNA isolated is a limitation. Not more that 1-3uL can be...

26 November 2013 1,754 8 View

Has anyone encountered any problems with abm good microRNA primers for quantification of mature miRNAs?

I've been trying to quantitate mature microRNAs in 293T cells with and without 'X'. 2ug of total RNA (isolated by Trizol method) is converted to cDNA using All-in one microRNA cDNA synthesis kit...

17 February 2021 8,986 2 View

Does anybody know the ways of homogeneous ripening of Mung bean crop?

Some crops (Mung bean) are matured at different times i.e., harvested at several times that's why the labour cost of the farmer is high. So, could you please, is there any ways or chemicals are...

12 February 2021 294 4 View

How much could knowing the maturity level of an organization contribute to a given process?

The doubt is raised because there are several methodologies to estimate the degree of maturity of certain processes, such as computerization, communication, human resources, among others, and...

03 February 2021 4,753 7 View

Does the subsoil have an effect on the spread of weeds whose roots are more than 35 cm deep?

For weeds whose roots deepen more than the level of subsoil, they may be affected by this layer, for example, determined by height and growth, as well as limited to flower production and seeds.

01 February 2021 1,940 4 View

Is there any correlation between mature miRNAs and their precursors?

I have a series of RNA-seq datasets that their average spot length is about 90bp. (don't include small RNA reads like mature miRNAs). I'd like to do "Gene" and "precursor miRNAs" expression...

07 December 2020 7,744 3 View

Hello every one! How can i design the primer for mature microRNA for RT-PCR quantification?

I am going to validate some microRNA genes in two developmental stages. But i found the method for miRNA primer designing is totally change compering to mRNA and LncRNA primers designing. Please...

03 December 2020 4,555 6 View

R script statistics - Effect of 2 variables on another?

There is data about the location and characteristics of plots of Oilseed Rape on several farms, as well as the relative abundance of different pollinator species counted at each plot. The...

25 November 2020 3,203 1 View

Is there a database of photos with insect pollinators on flowers of plants?

I would like to know if there is a database of photos with insect pollinators on flowers. The insects must be located on flowers of plants. I am interested in pollinators encountered in Europe,...

04 November 2020 2,219 6 View

How to isolate proteins solubilized in ethanol?

hi, i'm conducting a study on wheat. I extracted the gliadins from these cereals and now they are solubilized in 60% ethanol. Is there a method of eliminating ethanol (other than boiling) that...

25 October 2020 8,506 3 View

How can you order the clasification in CORINE legends, from a year to another, in type of vegetation?

For example, CORINE clasificate a zone of pasture one year, but in the next temporal clasification give crop of dry cereals, and preview clasification don't find. How can I order then?

20 October 2020 3,913 1 View