Is it possible to get genomic context data on bulk of accessions?

I'm trying to build local gene synteny to identify homologs between different species. To do that, I've been accessing each individual gene of interest, going into gene view in NCBI and...

04 November 2020 9,038 2 View

Can anyone give me a guide regarding vcf files?

I have a VCF file with SNPs using the IGV program, when uploading the file some SNPs that are observed in a particular gene in IGV, when verifying it in GDV or Ensembl do not match

10 August 2020 699 3 View

How a devoted health blockchain could effectively identify and track covid-19 patients while preserving the confidentiality of medical records?

Dear colleagues, I am currently working on a health's blockchain able of integrating informations relating to COVID-19 patients. The difficulty is to allow this blockchain to be a general...

21 April 2020 8,492 4 View

How to download citation network data from Web of Science (WoS)?

I want to do citation analysis on papers on ecnomics, but I don't know exactly how to get this data from Web of Science, though many sources say this is one of the very good datasets in this area....

16 March 2020 4,184 4 View

What is a good dataset and technique for citation analysis in Economics?

I would like to track the presence of some ideas in papers in economics, and I have been reading about citation network analysis and I have found some datasets (like the...

26 February 2020 2,848 1 View

Can someone explain about Different output of C/T vs A/G CSN1S2?

A NGS panel of truseq was performed, the SNP expected of CSN1S2, Ch 6, Position 87266177 are C/T, but we received A/G. There is an explain about that?

28 January 2020 1,118 2 View

Can you recommand Fc block antibody to use for immunophenotyping of rat stem cells (FACS)?

Dear RG community, I am looking for anti-rat FC blocking antibodies (CD16/C32) for the QC of a FACS immunophenotyping experiment. So far, I can only find anti-mouse Fc block from manufacturers...

04 December 2019 7,336 1 View

Where i can get the software ASSIGN 400ATF?

Can someone help me how i get the Assign 400ATF software for analysis of sequence of BoLA-DRB3 gene?

06 November 2017 7,938 2 View

What markers should I use for cardiac fibroblast?

11 February 2016 4,772 1 View

Does the primer used in a PCR may differ in bp size in electrophoresis?

We are performed an electrophoresis with primers taken from a paper based on hemiparasites samples. The posterior electrophoresis was different from the original work 160bp vs 700bp in several...

27 October 2015 4,544 6 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View

Can I use a protein-devoid condition as my negative control in ELISA?

Hello, I am running a sandwich-type ELISA where I am trying to inhibit a protein-protein interaction using small molecules. For each experiment, I determine my Z' (Z-prime) to determine the...

23 February 2021 7,429 1 View