There have been a few studies investigating the potential association between the CAPN13 gene and meat quality in cattle. CAPN13, also known as calpain 13, is a member of the calpain family of calcium-dependent cysteine proteases, which are involved in muscle protein degradation.
One study published in the journal Meat Science in 2015 investigated the association between the CAPN13 gene and meat quality traits in Chinese Simmental cattle. The study found that certain variations of the CAPN13 gene were associated with marbling score, tenderness, and cooking loss. Specifically, a variation in the promoter region of the gene was associated with higher marbling score and tenderness, while a variation in the coding region of the gene was associated with higher cooking loss.
Another study published in the journal Gene in 2018 investigated the genetic variation and expression of the CAPN13 gene in different beef cattle breeds. The study found that certain variations of the gene were associated with differences in meat color and tenderness. The study also found that the expression of the CAPN13 gene was higher in the longissimus muscle of Angus cattle compared to Simmental cattle, which may contribute to differences in meat quality between these breeds.
Overall, these studies suggest that the CAPN13 gene may play a role in meat quality traits in cattle, particularly in marbling, tenderness, and cooking loss. However, further research is needed to confirm these findings and to better understand the mechanisms by which the CAPN13 gene affects meat quality.
You can explore this gene from NCBI database. If you lucky, you will find this gene sequence from Bos indicus, Bos taurus dan ther crossing. Please select "gene" in the menu and click Bis taurus CAPN13. If you get many varians of bovine CAPN13 gene sequences, you can copy it one by one and use them for alignment with BioEdit software. If you find any mutation sites among bovine CAPN13 gene, you can designing a primer pairs to detect those mutation sites. You can try it and dont hessitate to contact me if you find any problem during analyse it. Thanks