I have KE-37 cells which I am trying to grow up to do centrosome isolation, however the cells aren't thriving. Any recommendations for culture conditions?
First, test the cells for Mycoplasma contamination and then follow the culture conditions recommended by DSMZ: http://www.dsmz.de/catalogues/details/culture/ACC-46.html?tx_dsmzresources_pi5[returnPid]=192