I would like to know if any of you have sonicated the cell line K562 with Bioruptor for ChIP. How many cycles do we need to properly sonicate this cell line?
thanks for your answer, bit in Orfeo to perform chromatin inmunoprecipitation (ChIP), I need to sonicate the cells to open the chromatin. I rutinelly use the bioruptor to Sonicate other human cancer cell lines and it works fine. I just wanted to know how many cycles I need to use with K562 cells.