Lara I. De Llobet

4 Questions 10 Answers 0 Followers

Questions related from Lara I. De Llobet

K562 cell line sonication (with bioruptor): how many cycles to use?

Hi, I would like to know if any of you have sonicated the cell line K562 with Bioruptor for ChIP. How many cycles do we need to properly sonicate this cell line?

07 March 2019 7,172 2 View

Is there any difference in culturing MCF10A cell line with FBS instead of Horse Serum?

I would like to know if there is any difference in culturing MCF10A cell line with FBS instead of Horse Serum. I've seen several protocols (including Brugge's lab) culturing the cell line with...

22 January 2016 9,929 15 View

With mammary cell line MCF10A, is there a big difference in the cell growth if we do not add cholera toxin to the culture medium?

I would like to know if someone working with MCF10A cells can help me to know if adding or not the cholera toxin to the culture growth medium fro MCF10A cells makes a big difference in the cell...

13 July 2015 1,126 6 View

Why do I have a high percentage of eosinophils when isolating PMNLs from whole blood samples?

I have performed a PMNL isolation from human whole blood (dextran sedimentation, ficoll-paque density gradient centrifugation, red blood cell (RBC) lysis). When I have checked the percentages of...

25 September 2014 1,410 5 View