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Questions related from Lara I. De Llobet
Hi, I would like to know if any of you have sonicated the cell line K562 with Bioruptor for ChIP. How many cycles do we need to properly sonicate this cell line?
07 March 2019 7,170 2 View
I would like to know if there is any difference in culturing MCF10A cell line with FBS instead of Horse Serum. I've seen several protocols (including Brugge's lab) culturing the cell line with...
22 January 2016 9,927 15 View
I would like to know if someone working with MCF10A cells can help me to know if adding or not the cholera toxin to the culture growth medium fro MCF10A cells makes a big difference in the cell...
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I have performed a PMNL isolation from human whole blood (dextran sedimentation, ficoll-paque density gradient centrifugation, red blood cell (RBC) lysis). When I have checked the percentages of...
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