Journal for microRNA research.

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Does anyone have experience with Co-IP using multiple antibodies at once?

I would like to know if anybody has ever tried performing co-immunoprecipitation using a mix of two or more antibodies against a partcular protein (I mean not separately, but together in one set...

06 July 2013 1,374 10 View

Consortia of similar journals?

There could be 1000s of journal in the field of biology, which a very broad subject. But certainly there could be limited number of journals for specific subjects e.g. crystallography,...

02 March 2013 7,679 10 View

Paper writing: before or after thesis writing?

Is it advisable for PhD students to write their research articles all at once at the end of their PhD thesis, while or after completing thesis writing or they should be written and published as...

31 December 2012 8,589 91 View

Does knowledge come only with the age or number of years one spends in a particular field?

Earlier I raised one question that what is the impact of a "review article" compared to a "research article" in scientific community. The topic was very well discussed but many of the topic...

31 December 2012 2,971 24 View

Does anybody know a nice working antibody to detect total sumoylation status in the protein preparation from heart or cell lysate?

I want to detect the sumoylation status of protein lysate prepared from mouse tissue and skeletal muscle cell line (C2C12 cells) lysate and I am still trying to figure out which single antibody...

11 December 2012 1,116 4 View

Is it possible that the transcriptional regulation of a given microRNA remains the same in different organs / tissues / cells?

I am wondering if every microRNA is regulated by the same transcription factors and regulatory mechanisms in all cell types it is expressed or every cell type has independent regulatory mechanism...

11 December 2012 6,231 29 View

'Master' miRNA or 'super' miRNA?

miRNAs control various physiological and pathological processes, but what controls microRNAs? What controls their switch-on switch off? Is there any miRNA which can be termed as a "super" miRNA...

09 October 2012 6,004 13 View

Are pri-miRNA and pre-miRNA sequences coding for all validated mature microRNAs known?

There are almost 1000 or more microRNAs known for human / mouse / rat. I am wondering if the pri-miRNA and / or premiRNA sequences of all these microRNAs is also known? If yes, is there any...

09 October 2012 4,144 3 View

Human genes and disease database

Is there a database of human genes and their association with genes? I mean a database where you can search for a particular disease and get a list of genes dysregulated in that disease condition?...

09 October 2012 655 2 View

Prediction of microRNAs against a gene of interest

I know there are several databases where one can look for the miRNA targets. But does anybody know if there is any database or online tool to identify putative miRNAs against a given gene of...

09 October 2012 8,275 7 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Can MicroRNA cross the blood brain barrier?

In diseased conditions, many genes and gene products are visible in the bloodstream. So my question is whether we can find traces of microRNA which is involved in disease and disorder in blood serum?

24 February 2021 7,064 3 View

Are exogenous molecules transferred between human cells during doubling ?

Hello RG community, I wonderer whether exogenous molecules (e.g. reporter plasmids, miRNAs or other artificial molecules) are transferred between cultured human cells during their doubling ? So,...

21 February 2021 6,633 3 View

Has anyone encountered any problems with abm good microRNA primers for quantification of mature miRNAs?

I've been trying to quantitate mature microRNAs in 293T cells with and without 'X'. 2ug of total RNA (isolated by Trizol method) is converted to cDNA using All-in one microRNA cDNA synthesis kit...

17 February 2021 8,986 2 View

What is the best way to fix plasma derived miRNA for subsequent cDNA synthesis?

I need to quantify blood plasma derived miRNA through q-RT-PCR. For this I need to fix RNA content in my cDNA reaction. What is the best way to do this? Currently, I take readings of my plasma...

03 February 2021 9,330 3 View

What is the general miRNA yield one gets from the Qiagen miRNEASY serum/plasma advanced kit?

I am using the Qiagen miRNEASY serum/plasma advanced kit, using 400ul of frozen plasma. What is the yield you get on average? I am using nanodrop currently for quantification. I am getting...

02 February 2021 8,806 2 View

Which miRNA arm is a right target for analysis, 3p or 5p?

I'm working on selecting mouse miRNAs to analyze and stumbling upon one confusing issue, some of the papers do not specify which arm they analyzed and they just state something like " miR-34a was...

16 January 2021 3,792 4 View

Is negative controls necessary for western blotting?

I am trying to confirm my miRNA target by western blotting. I have a ds mimic and a ss inhibitor of my miRNA. Is it necessary to use negative controls for mimic and inhibitor? If yes, how can I...

09 January 2021 2,320 3 View

Can I predict miRNA by knowing the mRNA target?

Hello! I am trying to predict in silico the miRNA by knowing the target. Most of published research starts by knowing the miRNA and predicts the target using online tools. Are you familiar with...

31 December 2020 7,433 5 View

What are the possible drawbacks of using miRNA sponge to enhance the specificity of gene expression?

I am searching for miRNA repeats put between WPRE and 3'LTR of my lentivirus, in order to enhance the specificity of transgene expression in human neuron cells. But if clinically translation of...

26 December 2020 9,921 2 View