03 March 2013 8 4K Report

Is there any journal dedicated specially to microRNA research, and accepts research and review articles associated with microRNA?

More Ashraf Rangrez's questions See All
Does anyone have experience with Co-IP using multiple antibodies at once?

I would like to know if anybody has ever tried performing co-immunoprecipitation using a mix of two or more antibodies against a partcular protein (I mean not separately, but together in one set...

06 July 2013 1,515 10 View

Consortia of similar journals?

There could be 1000s of journal in the field of biology, which a very broad subject. But certainly there could be limited number of journals for specific subjects e.g. crystallography,...

02 March 2013 7,815 10 View

Paper writing: before or after thesis writing?

Is it advisable for PhD students to write their research articles all at once at the end of their PhD thesis, while or after completing thesis writing or they should be written and published as...

31 December 2012 8,735 91 View

Does knowledge come only with the age or number of years one spends in a particular field?

Earlier I raised one question that what is the impact of a "review article" compared to a "research article" in scientific community. The topic was very well discussed but many of the topic...

31 December 2012 3,186 24 View

Does anybody know a nice working antibody to detect total sumoylation status in the protein preparation from heart or cell lysate?

I want to detect the sumoylation status of protein lysate prepared from mouse tissue and skeletal muscle cell line (C2C12 cells) lysate and I am still trying to figure out which single antibody...

11 December 2012 1,253 4 View

Is it possible that the transcriptional regulation of a given microRNA remains the same in different organs / tissues / cells?

I am wondering if every microRNA is regulated by the same transcription factors and regulatory mechanisms in all cell types it is expressed or every cell type has independent regulatory mechanism...

11 December 2012 6,345 29 View

'Master' miRNA or 'super' miRNA?

miRNAs control various physiological and pathological processes, but what controls microRNAs? What controls their switch-on switch off? Is there any miRNA which can be termed as a "super" miRNA...

09 October 2012 6,137 13 View

Human genes and disease database

Is there a database of human genes and their association with genes? I mean a database where you can search for a particular disease and get a list of genes dysregulated in that disease condition?...

09 October 2012 779 2 View

Are pri-miRNA and pre-miRNA sequences coding for all validated mature microRNAs known?

There are almost 1000 or more microRNAs known for human / mouse / rat. I am wondering if the pri-miRNA and / or premiRNA sequences of all these microRNAs is also known? If yes, is there any...

09 October 2012 4,275 3 View

Prediction of microRNAs against a gene of interest

I know there are several databases where one can look for the miRNA targets. But does anybody know if there is any database or online tool to identify putative miRNAs against a given gene of...

09 October 2012 8,403 7 View

Similar questions and discussions
Anyone having idea about VN primer for miRNA primer design ?

How to design VN primer to attach with universal reverse primer

05 August 2024 2,116 3 View

Which modeling software and force field suitable for miRNA-mRNA molecular simulation?

Hello everyone, I am currently working on miRNA- mRNA interaction study. Further, I would like to do MD simulations for the miRNA-mRNA models. For the Simulation, I have modelled this complex...

24 June 2024 3,507 2 View

Which is the best small Extracellulare Vesicles (sEVs) concentration to obtain enough RNA for miRNA analysis?

Hello to everyone, I'm trying to extrtact total RNA from sEVs. I made a first experiment starting from 2x10^10 sEVs quantified by NTA but the RNA obtained was very low (under 20ng/ml) Do you have...

18 June 2024 2,799 4 View

What is the best way to synthesize cDNA of miRNA; using poly A kit or stem loop PCR?

Recently, I have been trying to quantify the expression of miRNA. I have extracted the miRNA. However, I am not clear about using the cDNA synthesis kit usually there are two kits providing by...

16 June 2024 9,723 2 View

Extraction RNA with miRVANA kit?

Hello everyone, I would like to know if any of you ever tried to assess the yeld of RNA extraction with the mirVana™ miRNA Isolation Kit, without phenol from ThermoFisher. I am experincing some...

06 June 2024 5,715 4 View

I need guidance how to design primers for miRNA?

The size miRNA is 89 bps and the primers I have designed (using traditional method for designing gene primers) should give an amplified product of 71 bps. But I am getting product of higher...

23 May 2024 7,570 3 View

Should the target miRNA level decrease after the miRNA inhibitor transfection when checked with qPCR??

I have a question regarding target miRNA expression after transfection with antisense miRNA (miRNA inhibitor). I understand that the expression of the target miRNA will increase after mimic...

20 May 2024 5,920 4 View

Webserver for identifying activation and repression of TFs, genes, and miRNAs?

Hello. Is there any webserver where I can input a set of transcription factors, genes, and miRNAs, and it can provide me with statistical data on how TFs either activate or repress genes and...

18 May 2024 5,848 1 View

Does anybody face problems with total RNA isolated after miRNA mimic and inhibitor transfection?

Hello all. I have recently been trying to isolate total RNA after transfection of miRNA mimics and antisense inhibitors in mammalian cells. However, the RNA isolated through the trizol method...

13 May 2024 4,935 7 View

I would like to work on microRNA detect colorectal cancer. Which type of microRNA type micRNA124,micro143 how I can make primer design for it ?

would like to work on microRNA to detect colorectal cancer. I will use the type of microRNA type micRNA124,micro143 how I can make primer design for it and how I can choose the primer ?

07 May 2024 8,812 2 View

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