2 Questions 7 Answers 0 Followers
Questions related from Samaneh Tousi
I want to fuse a fragment with 600bp length to a 2.4 kb fragment with very stable secondary structures. Overlaping primers have 42 bp length (54.8% GC content and total Tm=69ºC), their 3’ end...
01 January 2015 6,575 8 View
I’m designing a set primer (with melting temp about 60°c) for PCR of a genomic fragment (5 kb). In this region of genomic DNA, there are several very stable secondary structures (about ΔG = -190...
06 June 2014 6,972 9 View
