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Questions related from Linoy Israel
Hi I'm looking for protocol to image human PBMCs on confocal microscopy with Mitotracker deep red for mitochindria staining. How long the recovery time should be(after thawing them)? I looked...
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I'm looking for a protocol for doing fluoresence microscopy to see the mitochondria shape/AR, on PBMCs from human blood?
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I want to measure the human plasma proteins concentration by nanodrop. Is regular water will be good? or I need to use nuclease-free water? or absolutely another material? Thank you!
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Hi I will be happy for your help. I'm doing real-time PCR with two primers called MT-TL1 and 18S, for mtDNA and houskeeping gene, and I got positive control on both NCT. I tried also different...
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Hi, I want to measure the copy number of mtDNA by qPCR by doing nDNA/mtDNA. This, of course, will be after DNA extraction. The problem is that I think that I will get also the mtDNA which inside...
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Hi! Does someone know how to clean lipoproteins from EVs? (from human plasma) We are trying to use iodixanol sucrose gradient (Optiprep), but we don't see EVs markers on the western blot after...
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Looking for protocol - MitoTimer in human PBMCs Is it possible to applied MitoTimer method on human peripheral blood mononuclear cells? I want to monitor biogenesis vs mitophagy processes. If...
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