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Questions related from Linoy Israel
I want to remove platelets from my human plasma samples to run after that qPCR (checking mtDNA/nuclear DNA copy number). Because of the known problem of over quantification if there are platelets...
09 November 2021 8,451 4 View
Hi I'm looking for protocol to image human PBMCs on confocal microscopy with Mitotracker deep red for mitochindria staining. How long the recovery time should be(after thawing them)? I looked...
11 November 2020 6,950 2 View
I'm looking for a protocol for doing fluoresence microscopy to see the mitochondria shape/AR, on PBMCs from human blood?
06 November 2020 9,135 3 View
Mitochondria witout crista (distrupted crista) or technical issue? I'm wondering what I see in my TEM images about the mitochodnria. I know there is some diseases where the cells can contain...
02 November 2020 3,004 4 View
I want to measure the human plasma proteins concentration by nanodrop. Is regular water will be good? or I need to use nuclease-free water? or absolutely another material? Thank you!
15 July 2020 984 0 View
Hello. I read that it's possible to compare the ct values of the nuclear gene to mtDNA gene. Does someone have experience with it? How do I need to plane my qPCR reaction? How I'm doing the...
15 July 2020 9,156 1 View
Hi I will be happy for your help. I'm doing real-time PCR with two primers called MT-TL1 and 18S, for mtDNA and houskeeping gene, and I got positive control on both NCT. I tried also different...
08 March 2020 4,185 4 View
Hi, I want to measure the copy number of mtDNA by qPCR by doing nDNA/mtDNA. This, of course, will be after DNA extraction. The problem is that I think that I will get also the mtDNA which inside...
28 January 2020 4,212 4 View
Hi! Does someone know how to clean lipoproteins from EVs? (from human plasma) We are trying to use iodixanol sucrose gradient (Optiprep), but we don't see EVs markers on the western blot after...
01 January 1970 5,540 1 View
Looking for protocol - MitoTimer in human PBMCs Is it possible to applied MitoTimer method on human peripheral blood mononuclear cells? I want to monitor biogenesis vs mitophagy processes. If...
01 January 1970 3,342 0 View
