Which nucleases can degrade G-quadruplex ?

I am interested in the effect of different nucleases on G-quadruplex DNA. I see examples in the literature where exonucleases or endonucleases release G-rich sequence which consequently form...

10 November 2018 781 2 View

Melting temperature (Tm) of G-quadruplex DNA?

I am using G-rich oligonucleotides to form corresponding G-quadruplex DNA using a standard protocol. Specifically, I am using 22mer HTelo and 22mer human oncogene promoter with Tm of 57.7 and 61.8...

09 October 2018 9,328 9 View

Sources for 5-HT2 receptors?

Is anyone aware of sources to get membranes expressing 5-HT receptors for in vitro assays ?

31 December 2017 5,317 2 View

Software for molecular docking with oligonucleotides?

I am trying to do molecular docking studies for metal complexes using crystal structure of oligonucleotide from PDB 2O1I. However, trying to perform it with Swissdock or Molegro failed because...

11 December 2017 924 3 View

Supercoiled plasmid DNA vs relaxed DNA differential binding ?

Few fluorescent dyes are reported to differentiate between supercoiled plasmid DNA and relaxed DNA by differential binding of the dye upon DNA relaxation ( ex: Picogreen). I was wondering how to...

11 December 2017 3,251 3 View

Quantification of ionic and covalent character ?

Are there any simple computational methods to quantify ionic and covalent characters of bond that are considered intermediate between ionic and covalent?. I thought of estimation of bond length...

09 October 2017 9,475 3 View

Solubility of Cobalt (II) compounds ?

I am wondering if there is any explanation for the dependence of solubility of Co(II) inorganic compounds in water on the counter ion. In particular, cobalt (II) cyanide is completely insoluble in...

08 September 2017 3,959 9 View

DLS measurements analysis ??

08 September 2017 9,897 4 View

Experimental proof for intermolecular charge transfer in metal complexes

For some reported metal complexes as anion sensors in the literature, the proposed mechanism is that upon binding of the anion to the metal center, the extent of ICT from ligand to metal is...

07 August 2017 1,000 7 View

Fluorescent dye to differentiate between supercoiled and relaxed DNA ?

Are there any well established fluorescent dyes (alternatively to PicoGreen) that can differentiate between supercoiled and relaxed DNA, so that they are able to measure the difference in...

06 July 2017 1,410 3 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

What do I do with DLS peaks that are from the buffer?

I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...

01 March 2021 9,015 2 View

Bought HUVEC from Invitrgen.need to revive it.expected it is cryofreezed in DMSO.Do I need to remove the DMSO for plating the cell for the 1st time?

After I cryofreeze the cell do I need to remove the DMSO for culturing the cell for next passages .

28 February 2021 7,738 3 View

POlymer+DMSO solution and moisture??

What could be the reason of moisture at the sides of Vial when I am preparing my polmyer+DMSO solution for membrane fabrication? What etiquettes I am possibly missing here?

28 February 2021 6,516 5 View

Why initial concentration of Pb not the same as calculated one when testing using AAS?

I am currently doing research on the adsorption of heavy metal using magnetic reduce graphene oxide. I use AAS to determine the concentration of the heavy metal. I prepare the Pb solution with...

26 February 2021 9,580 4 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View