17 Questions 26 Answers 0 Followers
Questions related from Moustafa Gabr
I am interested in the effect of different nucleases on G-quadruplex DNA. I see examples in the literature where exonucleases or endonucleases release G-rich sequence which consequently form...
11 November 2018 867 2 View
I am using G-rich oligonucleotides to form corresponding G-quadruplex DNA using a standard protocol. Specifically, I am using 22mer HTelo and 22mer human oncogene promoter with Tm of 57.7 and 61.8...
10 October 2018 9,423 9 View
Is anyone aware of sources to get membranes expressing 5-HT receptors for in vitro assays ?
01 January 2018 5,413 2 View
Few fluorescent dyes are reported to differentiate between supercoiled plasmid DNA and relaxed DNA by differential binding of the dye upon DNA relaxation ( ex: Picogreen). I was wondering how to...
12 December 2017 3,413 3 View
I am trying to do molecular docking studies for metal complexes using crystal structure of oligonucleotide from PDB 2O1I. However, trying to perform it with Swissdock or Molegro failed because...
12 December 2017 1,014 3 View
I am considering to perform ITC experiment for small molecule - peptide interaction. Both small molecule and peptide are insoluble in PBS buffer. I dissolve them in DMSO first then dilute with PBS...
11 November 2017 310 2 View
Are there any simple computational methods to quantify ionic and covalent characters of bond that are considered intermediate between ionic and covalent?. I thought of estimation of bond length...
10 October 2017 9,567 3 View
I am wondering if there is any explanation for the dependence of solubility of Co(II) inorganic compounds in water on the counter ion. In particular, cobalt (II) cyanide is completely insoluble in...
09 September 2017 4,053 9 View
I am using Malvern Zetasizer Nano ZS instrument to perform DLS measurements. I am getting this statement at the end of all my measurements (means with ± 1 SD) like in the picture attached. Does...
09 September 2017 9,997 4 View
For some reported metal complexes as anion sensors in the literature, the proposed mechanism is that upon binding of the anion to the metal center, the extent of ICT from ligand to metal is...
08 August 2017 1,091 7 View
Are there any well established fluorescent dyes (alternatively to PicoGreen) that can differentiate between supercoiled and relaxed DNA, so that they are able to measure the difference in...
07 July 2017 1,564 3 View
Tuning luminescence of platinum complexes is well established by variation of cyclometalating ligand. The most common trend I find in literature is a red shift in the emission upon introducing...
06 June 2017 4,352 3 View
Most of the examples I found in literature for constructing Stern Volmer plots to study interaction with HSA or BSA relies on quenching effect of the probe on the emission from tryptophan residue....
05 May 2017 2,576 3 View
HSA has a molecular mass of 66.5 kDa. When I did DLS measurements for pure HSA I was expecting to see large-sized particles. However, I ended up having 8-9 nm particles which I later found it does...
04 April 2017 3,577 6 View
I am using thiophosgene to convert primary aromatic amine to isothiocyanate in a biphasic solvent system (chloroform/water). Under these conditions tert-butyl ester in my starting material is...
11 November 2016 7,362 7 View
Are there any in vitro assays to evaluate or predict the ability of small molecules to cross blood brain barrier ?
11 November 2016 2,320 5 View
I am using ThT and Congo red assays to monitor Amyloid Beta 1-42 aggregation. It worked fine before, but now I am evaluating compounds with overlapping UV absorabance to these dyes. So any...
11 November 2016 8,153 8 View
